Grafenoxid (GO) ar en lovande kandidat som nano-tillsats i medicinska byggnadsstallningar for benregenerering. GO kan forbattra den biologiska kompatibiliteten och osteogena prestandan hos polymerbaserade byggstallningar, och ocksa vasentligt bidra till forbattringen av materialets mekaniska egenskaper. I detta arbete ympades nano-grafenoxid (nGO) kovalent pa ytan av poly (e-kaprolakton) (PCL) genom att fdrst modifiera polymerytan via aminolys. Med anvandning av 1,6-hexandiamin / isopropanol infordes fria amingrupper framgangsrikt pa PCL-ytan for efterfoljande immobilisering av nGO. En optimerad ympningsprocess utvecklades via en losningsmedelsassisterad metod med vatten som losningsmedel for att kovalent binda nGO pa ytan av PCL byggnadsstallningar. De initiala nGO koncentrationerna var 0,5 och 1 mg / ml. fourier-transform infrarodspektroskopi (FTIR) och termogravimetrisk analys (TGA) verifierade bindningen mellan de funktionella gruppema pa nGO och de fria aminema. Svepelektronmikroskopi (SEM) visade en homogen fordelning av nGO pa ytan av de porosa byggnadsstallningarna. De mekaniska testema som utfordes demonstrerade · en 50 och 21 % okning av kompressionsstyrkan :for byggnadsstallningarna ympade med de initiala nGO-koncentrationema pa 0,5 och 1 mg / ml. In vitro-mineraliseringstester visade bildandet av mineralfallningar pa ytan av byggnadsstallningama som okade i storlek med hogre nGO-halt. A ven nGO: s potential som nano-barare av ett antibiotikum studerades i detta arbete. Pa grund av sitt overflod av kemiska funktionaliteter kan nGO effektivt adsorbera foreningar genom olika sekundara interaktioner. I denna studie optimerades dessa sekundara interaktioner genom att reglera losningens pH for maximal adsorption av ciprofloxacin, ett bredspektrum antibiotikum som anvands vid behandling av osteomyelit. Ciprofloxacin befanns kunna adsorberas starkast i sin katjonform vid pH 5, dar 1t-1t elektrondonatoracceptor (EDA) -interaktioner dominerar. Sammanfattningsvis bekraftar de resultat som presenteras i detta arbete potentialen hos nGO som egenskapsforbattrare och lakemedelsbarare i applikationer inom vavnadsregenerering. / Graphene oxide (GO) is a promising candidate as nano-filler material in scaffolds for bone regeneration. It has been demonstrated to enhance the biological compatibility and osteogenic performance of polymer-based scaffolds, aside from its substantial contribution to the improvement of the material's mechanical properties. In this work, nano-graphene oxide (nGO) was covalently grafted to the surface of poly( e-caprolactone) (PCL) by first modifying the polymer surface via aminolysis. Using 1,6-hexanediamine/isopropanol, free amine groups were successfully introduced to the PCL surface for the subsequent immobilization of nGO. An optimized grafting pathway, which implements the solvent-assisted method and uses water as a solvent, was developed to covalently attach nGO using initial concentrations of 0.5 and 1 mg/mL. Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) both verified the successful attachment of nGO through the free amines. Scanning electron microscopy (SEM) depicts a homogeneous dispersion of nGO over the polymer matrix. Mechanical tests were performed and demonstrate a 50 and 21 % increase in compressive strength for the scaffolds grafted using initial nGO concentrations of 0.5 and 1 mglmL. In vitro mineralization tests showed the formation of mineral precipitates on the surface of the scaffolds that increased in size with higher nGO content. The potential of nGO as a nano-carrier of an antibiotic drug was also explored in this work. As it comprises of an abundance of chemical functionalities, nGO is able to efficiently adsorb compounds through various secondary interactions. In this study, these secondary interactions were optimized by controlling the solution pH for the maximum adsorption of ciprofloxacin, a broad-spectrum antibiotic used in the treatment of osteomyelitis. Ciprofloxacin was found to be adsorbed most strongly in its cationic form at pH 5, in which 1t-1t electron-donor acceptor (EDA) interactions predominate. Overall, the results presented in this work validate the potential of nGO as nano-enhancer and drug carrier in tissue engineering scaffold applications.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:kth-231268 |
| Date | January 2018 |
| Creators | Jenevieve Linell, Yao |
| Publisher | KTH, Skolan för kemi, bioteknologi och hälsa (CBH) |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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