The association of members of the enterovirus family with pregnancy complications up
to miscarriages is under discussion. Here, infection of two different human induced pluripotent
stem cell (iPSC) lines and iPSC-derived primary germ-layer cells with coxsackievirus B3 (CVB3) was
characterized as an in vitro cell culture model for very early human development. Transcriptomic
analysis of iPSC lines infected with recombinant CVB3 expressing enhanced green fluorescent protein
(EGFP) revealed a reduction in the expression of pluripotency genes besides an enhancement of
genes involved in RNA metabolism. The initial distribution of CVB3-EGFP-positive cells within
iPSC colonies correlated with the distribution of its receptor coxsackie- and adenovirus receptor
(CAR). Application of anti-CAR blocking antibodies supported the requirement of CAR, but not of
the co-receptor decay-accelerating factor (DAF) for infection of iPSC lines. Among iPSC-derived
germ-layer cells, mesodermal cells were especially vulnerable to CVB3-EGFP infection. Our data
implicate further consideration of members of the enterovirus family in the screening program of
human pregnancies. Furthermore, iPSCs with their differentiation capacity into cell populations of
relevant viral target organs could offer a reliable screening approach for therapeutic intervention and
for assessment of organ-specific enterovirus virulence.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:88927 |
| Date | 10 January 2024 |
| Creators | Böhnke, Janik, Pinkert, Sandra, Schmidt, Maria, Binder, Hans, Bilz, Nicole Christin, Jung, Matthias, Reibetanz, Uta, Beling, Antje, Rujescu, Dan, Claus, Claudia |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1220, 10.3390/ijms22031220 |
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