The aim of the study was to investigate if PRIMA-1, a drug that reactivate the tumour suppressor gene p53, will have a synergistic effect when used in combination with γ-irradiation in a human small cell lung cancer cell line (U-1690), which show a low-dose hypersensitivity in clonogenic survival experiments. Clonogenic cell survival after incubation with PRIMA-1 alone in concentrations from 0.1 to 30 µM or in combination with γ-irradiation with a 137Cs source was performed. The standard LQ model, and the repairable-conditionally repairable damage model, the RCR model, were fitted to the experimental data. PRIMA-1 showed a time and concentration dependent cytotoxic, and 10 µM killed 60 % of cells during 72 hours incubation. The cells show low-dose hypersensitivity calculated with the RCR model. The combined treatment with 8 µM PRIMA-1 had a synergistic effect with irradiation doses from 2 Gy and above. The overall conclusion is that a combination with PRIMA-1 and irradiation is beneficial to achieve the best effect.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:kth-172448 |
| Date | January 2015 |
| Creators | ORSAN EGNERFORS, SOLWEIG |
| Publisher | KTH, Skolan för kemivetenskap (CHE) |
| Source Sets | DiVA Archive at Upsalla University |
| Language | Swedish |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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