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Previous issue date: 2013-03-19 / Panax ginseng CA Meyer (Araliaceae) is a herbaceous plant widely used in China,
South Korea, Japan and other Asian countries for the treatment of various diseases
micro circulatory, cerebrovascular, among others, representing one of the drugs used by
older man. It has over 30 biologically active ginsenosides with different
pharmacological and behavioral effects and inhibitory effect on the NMDA receptor.
The amino acid glycine is a co-agonist of the NMDA receptor, activating this receptor.
At the cellular level, ketamine is widely known to be NMDA receptor antagonist. The
aim of this study was to evaluate the general activity in the open field, and anxiety in
elevated plus maze, mice treated with P. ginseng compared with the action of ketamine
and glycine, to better understand the action of this herbal medicine at the NMDA
receptor. We used 66 adult male rats were divided into six groups: a positive control,
treated for 30 days with water by gavage, who received glycine (500mg/kg; po) on days
7, 14, 21 and 28 of treatment, one hour before of behavioral assessment, a negative
control was treated for 30 days with water by gavage received ketamine (5mg/kg, ip) on
days 7, 14, 21 and 28 of treatment, one hour prior to behavioral evaluation, three
experimental groups, receiving 100, 200 or 300 mg / kg P. ginseng by gavage for 30
days and one group treated solely with white water, and is also administered 1 ml of
water by gavage one hour prior to behavioral evaluation. Animal behavior in these three
groups was also examined on days 7, 14, 21 and 28 of treatment. On day 30 of
treatment, the animals were anesthetized with thiopental (70mg/kg) for blood collection
and after euthanasia, withdrawal of various organs. There were no changes in weight
and body weight gain and weight reasons in organ / body weight. However the
consumption of water and food values showed a significant increase. Serum levels of
AST was increased in a dose-dependently in the animals treated with doses of P.
ginseng, glycine and ketamine as compared to the blank group. Unlike creatinine levels
proved to be decreased in all treated groups when compared with white. However, the
level of urea in these groups was reduced and no changes were observed in the ALT
parameter. Histopathological examination revealed no changes in cell morphology in
different tissues. There were no behavioral changes in the elevated plus maze and few
changes were observed in the open field, animals treated with P. ginseng, glycine and
ketamine when compared to white. These data suggest that the doses of P. ginseng
employed were unable to induce general toxicity in rats treated for 30 days and also
shows that the general behavior of mice treated with P. ginseng was slightly different
from that observed in animals treated with ketamine and glycine. Finally, the study on
the elevated plus maze showed that the extract of P. ginseng showed no anxiolytic or
anxiogenic action / Panax ginseng C.A. Meyer (Araliaceae) ? uma planta herb?cea muito usada na China,
Cor?ia do Sul, Jap?o e outros pa?ses da ?sia no tratamento de v?rias doen?as micro
circulat?rias, vasculares cerebrais, entre outras. Possui mais de 30 ginsenos?deos, que
inibem o receptor NMDA, provocando diferentes efeitos farmacol?gicos e
comportamentais. O objetivo do presente estudo foi avaliar a atividade geral, no campo
aberto, e a ansiedade, no labirinto em cruz elevado, de ratos tratados com P. ginseng.
Ratos tratados com ketamina (antagonista do receptor NMDA) e com glicina (coagonista
do receptor NMDA), foram tamb?m empregados para melhor entendimento do
mecanismo de a??o desse fitoter?pico. Foram utilizados 66 ratos machos adultos,
divididos em seis grupos: um controle positivo (n=12), tratado durante 30 dias com
?gua por gavagem, que recebeu glicina (500mg/kg; v.o.) nos dias 7, 14, 21 e 28 de
tratamento, uma hora antes da avalia??o comportamental; um controle negativo (n=12),
tratado durante 30 dias com ?gua por gavagem, que recebeu ketamina (5mg/kg; i.p.) nos
dias 7, 14, 21 e 28 de tratamento, uma hora antes da avalia??o comportamental; tr?s
grupos experimentais (n=12), que receberam 100, 200 ou 300 mg/kg de P. ginseng, por
gavagem, durante 30 dias e um grupo branco (n=6) tratado exclusivamente com ?gua,
sendo tamb?m administrado 1mL de ?gua por gavagem uma hora antes da avalia??o
comportamental. O comportamento animal nesses grupos tamb?m foi analisado nos dias
7, 14, 21 e 28 de tratamento. No dia 30 de tratamento os animais foram anestesiados
para coleta de sangue e retirada de ?rg?os diversos, que tiveram seus pesos anotados e
por??es foram coletadas para estudo histopatol?gico. N?o foram observadas altera??es
no peso e ganho de peso corporal entre os diversos grupos nem nas raz?es peso
?rg?o/peso corporal calculadas. Nos animais tratados com P. ginseng, ketamina e
glicina o consumo de ?gua e de ra??o e as concentra??es s?ricas de AST revelaram estar
aumentadas em compara??o com grupo branco. Entretanto, os animais tratados com as
tr?s doses de P. ginseng, ketamina e glicina apresentaram n?veis reduzidos de creatinina
e ureia quando comparados com o grupo branco. N?o foram observadas altera??es no
par?metro ALT. O estudo histopatol?gico revelou aus?ncia de altera??es na morfologia
celular nos diversos tecidos analisados. N?o foram encontradas altera??es
comportamentais no labirinto em cruz elevado e poucas altera??es foram observadas
nos animais tratados com P. ginseng, glicina e ketamina quando comparados com o
grupo branco, no campo aberto. Esses dados sugerem que as doses de P. ginseng
empregadas n?o foram capazes de provocar toxicidade geral em ratos tratados por 30
dias e revela tamb?m que o comportamento geral dos ratos tratados com P. ginseng foi
pouco diferente daquele observado nos animais tratados com glicina e ketamina. Por
fim, o estudo no labirinto em cruz elevado mostrou que o extrato de P. ginseng n?o
apresentou a??o ansiog?nica nem ansiol?tica nas condi??es experimentais adotadas
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13495 |
| Date | 19 March 2013 |
| Creators | Matos, Ana Laura de Souza Almeida |
| Contributors | CPF:03065039648, http://lattes.cnpq.br/2401319567715548, Schimieguel, Dulce Marta, CPF:65349598953, http://lattes.cnpq.br/6361482547166270, Kujbida, Paula da Silva, CPF:28974955881, http://lattes.cnpq.br/2285854300593624, Schwarz, Aline |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas, UFRN, BR, Bioan?lises e Medicamentos |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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