The sonic muscle of toadfish is the fastest vertebrate muscle ever measured, and
the rate of transport of Ca2+ and dissociation of cross-bridge are also fatest.
Parvalbumins are Ca2+-binding proteins present in vertebrate muscle, and they can aid
muscle relaxation. Several isoforms of parvalbumin had been identified and presented
in variable proportion in different kinds of muscles (e.g. red muscle, white muscle and
pink muscle). Both male and female grunting toadfish (Allenbatrachus grunniens)
have intrinsic sonic muscles attached on swim bladders. The morphology of male and
female sonic muscle was compared, and no significant differences in both length,
width, thickness and weight were found. SDS-PAGE and western blotting were used
to determine the total parvalbumin expression and identify the parvalbumins from
sonic muscle and body white muscle. There were no significant differences in total
parvalbumin expression in sonic muscle and body white muscle. The result indicates
that there is no positive correlation between high content of parvalbumins and speed
of muscle relaxation. In native-PAGE, two and four parvalbumin isoforms were
identified from sonic muscle and body white muscle, respectively. The estimated size
of Parv1, Parv2 and Parv3 size in grunting toadfish¡¦s sonic muscle were 10kDa,
10.5kDa and 10.5kDa, respectively, and the isoelectric points of Parv1, Parv2 and
Parv3 in grunting toadfish were 4.77, 4.58 and 4.42, respectively. In the sonic muscle,
the major parvalbumin isoform was parvalbumin isoform 1 (Parv1), which comprised
more than 94% of total parvalbumin, and parvalbumin isoform 2 (Pav2) comprised
vi
only 5% of total parvalbumin content. In body white muscle, on the other hand, the
major isoform was parvalbumin isoform 2 (Parv2) which comprised 58% of toal
parvalbumin. Both Parv1 (with Parv1a) and Parv3 comprised about 20%.
Parvalbumin isoforms were be discussed. The result supports that Parv1 has a highest
effect on the relaxation of the grunting-toadfish¡¦s sonic muscle.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0705111-174344 |
| Date | 05 July 2011 |
| Creators | Hsieh, Fu-Ming |
| Contributors | Hurng-Wern Huang, Kuo-Hsun Chiu, Hin-Kiu Mok |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0705111-174344 |
| Rights | withheld, Copyright information available at source archive |
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