Biological, serological, and molecular characteristics
of the P4 pathotype of pea seedborne mosaic potyvirus
(PSbMV) were investigated. The characterization allowed P4
to be differentiated from other pathotypes of the same
virus.
P4 was distinguished from P1 on the basis of host
symptomatology. It induced varied, but often distinctive
symptoms in systemically-infected pea (Pisum sativum)
cultivars. P4 inoculation of indicator hosts, notably
Chenopodium album, resulted in the formation of local
lesions.
Pathotype P4 was seed transmitted at low frequencies
relative to P1. In ten selected pea cultivars, seed
transmission of P4 never exceeded 0.7%, while P1 was
transmitted at frequencies as high as 33%. In mixed
infections with P1, pathotype P4 was seed transmitted at 0-
2% as determined by P4-specific indirect ELISA. However,
limited polymerase chain reaction (PCR) data suggest that
P4 may be transmitted with P1 at higher rates than
indicated by ELISA data.
P4 was also aphid transmitted at relatively low rates.
Pea aphids (Acyrthosiphon pisum) allowed 3 or 5 min
acquisition access periods (AAP's) transmitted P4 at a
maximum rate of 16% (3 aphids/plant). P4 transmission
required short (1-7 min) AAP's and exhibited no bimodal
character within tested parameters.
P4 could be serologically distinguished from the other
pathotypes using monoclonal and polyclonal antibodies.
Tests with cross-reactive P4 antiserum suggest that P4 is
more closely related to P2 than P1. P4-specific antiserum
was derived by cross-absorbing diluted antiserum with P1-
infected pea sap to eliminate P1- reactive antibodies.
P1 and P4 RNA was detected and differentiated using
pathotype-specific oligonucleotide primers in conjunction
with PCR. Viral RNA sequences were amplified in pure
preparations as well as crude pea tissue (leaf, root,
pollen, and seed) extracts. / Graduation date: 1993
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/36166 |
| Date | 23 July 1992 |
| Creators | Kohnen, Paul D. |
| Contributors | Hampton, Richard O. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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