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Adrenomedullin, calcitonin gene-related peptide and endothelin-3 in mouse astrocyte cultures: actions and interactions.

by Chi Fung Yeung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 83-120). / Abstracts in English and Chinese. / abstract --- p.1 / table of contents --- p.3 / acknowledgments --- p.8 / declaration --- p.9 / list of figures and tables --- p.10 / list of abbreviations --- p.13 / Chapter chapter 1- --- general introduction --- p.15 / Chapter 1.1 --- VASOACTIVE PEPTIDES --- p.18 / Chapter 1.1.1 --- HISTORICAL BACKGROUND --- p.18 / Chapter 1.1.1.1 --- Adrenomedullin --- p.20 / Chapter 1.1.1.2 --- Calcitonin gene-related peptide --- p.21 / Chapter 1.1.1.3 --- Endothelin-3 --- p.22 / Chapter 1.1.2 --- "SYNTHESIS AND RELEASE OF ADRENOMEDULLIN, CALCITONIN GENE RELATED PEPTIDE AND ENDOTHELINS" / Chapter 1.1.2.1 --- SYNTHESIS AND RELEASE OF Adrenomeduulin --- p.24 / Chapter 1.1.2.2 --- SYNTHESIS AND RELEASE OF calcitonin gene related peptide --- p.25 / Chapter 1.1.2.3 --- SYNTHESIS AND RELEASE OF ENDOTHELINS --- p.25 / Chapter 1.1.3 --- "ADRENOMEDULLIN, CALCITONIN GENE RELATED PEPTIDE AND ENDOTHELINS IN THE CNS" --- p.26 / Chapter 1.1.4 --- "RECEPTORS OF ADRENOMEDULLIN, CALCITONIN GENE RELATED PEPTIDE AND ENDOTHELINS AND SIGNAL TRANSDUCTION" --- p.27 / Chapter 1.1.5 --- "BIOLOGICAL ACTIONS OF ADRENOMEDULLIN, CALCITONIN GENE RELATED PEPTIDE AND ENDOTHELINS" / Chapter 1.1.5.1 --- BIOLOGICAL ACTIONS OF ADRENOMEDULLIN --- p.30 / Chapter 1.1.5.1.1 --- haemodynamic effects of adrenomedullin --- p.30 / Chapter 1.1.5.1.2 --- Renal effects of Adrenomedullin --- p.31 / Chapter 1.1.5.1.3 --- Endocrine effects of Adrenomedullin --- p.31 / Chapter 1.1.5.1.4 --- Central effects of Adrenomedullin --- p.32 / Chapter 1.1.5.2 --- CALCITONIN GENE RELATED PEPTIDE / Chapter 1.1.5.2.1 --- haemodynamic effects of calcitonin gene related peptide --- p.33 / Chapter 1.1.5.2.2 --- Renal effects of calcitonin gene related peptide --- p.34 / Chapter 1.1.5.2.3 --- Endocrine effects of calcitonin gene related peptide --- p.34 / Chapter 1.1.5.2.4 --- Central effects of calcitonin gene related peptide --- p.35 / Chapter 1.1.5.3 --- ENDOTHELINS / Chapter 1.1.5.3.1 --- haemodyamic effects of endothelins --- p.36 / Chapter 1.1.5.3.2 --- Renal effects of Endothelins --- p.36 / Chapter 1.1.5.3.3 --- endocrine effects of endothelins --- p.37 / Chapter 1.1.5.3.4 --- Central effects of Endothelins --- p.37 / Chapter 1.1.6. --- PROLIFERATIVE OR ANTI-PROLIFERATIVE EFFECTS --- p.38 / Chapter 1.1.7 --- CLINICAL RELEVANCE OF VASOACTIVE PEPTIDES / Chapter 1.1.7.1 --- Clinical relevance of Adrenomedullin --- p.39 / Chapter 1.1.7.2 --- Clinical relevance of calcitonin gene related peptide --- p.40 / Chapter 1.1.7.3 --- Clinical relevance of Endothelins --- p.41 / Chapter 1.2 --- ASTROCYTES / Chapter 1.2.1 --- Historical background and astrocyte morphology --- p.42 / Chapter 1.2.2 --- Physiological roles of astrocytes --- p.44 / Chapter 1.2.3 --- Pathology of astrocytes --- p.45 / Chapter 1.3 --- NEUROPEPTIDE RECEPTORS AND ASTROCYTES / Chapter 1.3.1 --- Neuropeptide receptors on astrocytes --- p.47 / Chapter 1.3.2 --- Consequences of receptor activation --- p.49 / Chapter 1.4 --- AIMS OF THE THESIS --- p.50 / Chapter CHAPTER 2 --- "GENERAL MATERIALS AND METHODS, AND DATA ANALYSIS" / Chapter 2.1 --- MATERIALS / Chapter 2.1.1 --- ANIMALS --- p.54 / Chapter 2.1.2 --- PEPTIDE HORMONES --- p.54 / Chapter 2.1.3 --- ISOTOPES AND RADIOIMMUNOASSAY KITS --- p.54 / Chapter 2.1.4 --- CULTURE MATERIALS AND CHEMICALS --- p.55 / Chapter 2.1.5 --- PREPARATION OF MATERIALS / Chapter 2.1.5.1 --- Preparation of primary cultures of astrocytes --- p.55 / Chapter 2.1.5.2 --- Preparation of medium and binding buffer --- p.56 / Chapter 2.1.5.3 --- Preparation of 125I-labelled vasoactive peptides --- p.56 / Chapter 2.1.6 --- MEASUREMENT OF CELLULAR CYCLIC AMP --- p.57 / Chapter 2.1.7 --- DETERMINATION OF PROTEIN CONTENT OF CULTURED ASTROCYTES --- p.60 / Chapter 2.2 --- METHODS --- p.61 / Chapter 2.2.1 --- LIGAND BINDING: MEASUREMENT OF 125I-AM AND 125I-CGRP BINDING / Chapter 2.2.1.1 --- Binding kinetics --- p.61 / Chapter 2.2.1.2 --- Determination of specific binding of 125I-AM and 125I-CGRP --- p.61 / Chapter 2.2.1.3 --- Competition binding studies --- p.62 / Chapter 2.2.2 --- "BIOCHEMICAL INTERACTIONS BETWEEN ET3, AM, CGRP AND PKC-ANALOG" / Chapter 2.2.2.1 --- Determination of the production of cAMP in response to AM and CGRP --- p.62 / Chapter 2.2.2.2 --- Determination of the effect of PMA on AM and CGRP-dependent CAMP production --- p.63 / Chapter 2.2.2.3 --- Determination of the effect of the phorbol esters on AM and CGRP-dependent cAMP production --- p.63 / Chapter 2.2.2.4 --- Elucidation of antagonistic effect of staurosporine and Ro31-8220 --- p.64 / Chapter 2.2.2.5 --- Determination of the effects of ET-3 on AM and CGRP- dependent CAMP accumulation --- p.64 / Chapter 2.2.2.6 --- Determination of the effects of PKC inhIBition on ET-3 suppression of AM- and CGRP-induced CAMP responses --- p.64 / Chapter 2.2.2.7 --- Elucidation of antagonistic effect of cycloheximide --- p.65 / Chapter 2.3 --- STATISTIC ANALYSIS --- p.65 / Chapter CHAPTER 3 --- RESULTS / Chapter 3.1 --- BINDING KINETICS OF 125I-AM AND 125I-CGRP --- p.68 / Chapter 3.2 --- SPECIFIC BINDING OF 125I-AM AND 125I-CGRP --- p.68 / Chapter 3.3 --- COMPETITION BINDING --- p.68 / Chapter 3.4 --- DOSE RESPONSE OF ADRENOMEDULLIN AND CGRP- STIMULATED CAMP PRODUCTION --- p.69 / Chapter 3.5 --- EFFECTS OF PHORBOL ESTERS ON AM AND CGRP DEPENDENT CAMP ACCUMULATION --- p.69 / Chapter 3.6 --- EFFECT OF PMA ON AM AND CGRP-DEPENDENT CAMP ACCUMULATION --- p.70 / Chapter 3.7 --- EFFECT OF ET-3 ON AM AND CGRP-DEPENDENT CAMP ACCUMULATION --- p.70 / Chapter 3.8 --- "THE EFFECTS OF STAUROSPORINE, RO 31-8220" --- p.71 / Chapter 3.9 --- EFFECT OF CYCLOHEXIMIDE ON THE SUPPRESSIVE ACTION OF ET3 --- p.72 / Chapter CHAPTER 4 --- DISCUSSION --- p.73 / Chapter CHAPTER 5 --- GENERAL CONCLUSION --- p.80 / REFERENCES --- p.83 / APPENDIX-PUBLISHED PAPER

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323019
Date January 2000
ContributorsYeung, Chi Fung., Chinese University of Hong Kong Graduate School. Division of Medical Sciences.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, 121 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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