Screening For Antioxidant Activities Of Several Medicinal Plant Extracts And Their Effects On Glutathione-s-transferase Activity

Description

SCREENING FOR ANTIOXIDANT ACTIVITIES OF SEVERAL
MEDICINAL PLANT EXTRACTS AND
THEIR EFFECTS ON GLUTATHIONE-S-TRANSFERASE ACTIVITY


ABSTRACT

Sagdi&ccedil / oglu Celep, A. G&uuml / l&ccedil / in
Ph.D., Department of Biochemistry
Supervisor: Assoc. Prof. Nursen &Ccedil / oruh
May 2005, 154 pages

The consumption of fresh fruits, vegetables, and medicinal plants are known to be associated with a long life span and low incidence of oxidative stress related diseases such as Alzheimer&amp / #8217 / s, Parkinson&amp / #8217 / s, cancer, aging and cardiovascular diseases. Fitotherapeutic effects of medicinal plants is virtually attributable to their phenolic compounds with low cytotoxicity.

In this study, plants used in Anatolian folk medicine for their effects such as antiinflammatory, antiulcer, antipyretic, fertility, analgesic and aphrodisiac, namely Aesculus hippocastanum L., Papaver bracteatum L., Urtica urens L., Gundelia tournefortii L., Prangos ferulacea L., Chaerophyllum macropodum Boiss., Heracleum persicum Desf., Allium vineale L., Aconitum cochleare Woroschin, Rheum ribes L., Ferula rigidula DC., Rosa heckeliana Tratt, were screened for their antioxidative effects. Antioxidant characteristics of the specified plants were studied using lipid peroxidation inhibiton and DPPH radical scavenging methods.

Total phenolics content and their effects on glutathione-S-transferase activity of the plants were further investigated. Rheum ribes L, Ferula rigidula DC, Rosa heckeliana Tratt., Prangos ferulacea L. were found to be very effective antioxidants and also effective inhibitors for glutathione-S-transferase activities among the plants.

Rosa heckeliana Tratt. root extracts exhibited very high total phenolics content (0.7 mg/mg of extract) and antioxidant activity with IC50 values of 11.2 &micro / g/mL and 5.1 &micro / g/mL for DPPH scavenging and lipid peroxidation inhibition, respectively. Ferula rigidula DC was identified as the most potent inhibitor for glutathione-S-transferase activity, with IC50 values of 49 &micro / g/mL.

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1. http://etd.lib.metu.edu.tr/upload/3/12606166/index.pdf

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Identifer	oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/3/12606166/index.pdf
Date	01 May 2005
Creators	Sagdicoglu Celep, Gulcin Adviye
Contributors	Coruh, Nursen
Publisher	METU
Source Sets	Middle East Technical Univ.
Language	English
Detected Language	English
Type	Ph.D. Thesis
Format	text/pdf
Rights	To liberate the content for public access