This study was designed to determine the effect of inorganic phosphate in the growth media of A. vinelandii on the synthesis and accumulation of poly-β-hydroxybutyrate (PHB) and cellular morphology. Burk's media with no added phosphate and ten times normal phosphate resulted in altered cells, lower levels of protein, and decreased cell populations. PHB accumulation was much higher in the cultures with no added phosphate; however, the polymer granules differed in appearance from those of normal cells and had a decreased rate of utilization. The amount of PHB deposited in cells grown in media containing ten times the normal phosphate level was lower than that found in regular media.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc798266 |
| Date | 05 1900 |
| Creators | Collins, Patricia Ann |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vi, 49 leaves : ill., Text |
| Rights | Public, Collins, Patricia Ann, Copyright, Copyright is held by the author, unless otherwise noted. All rights |
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