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Arginine-specific negative regulation of Neurospora crassa arg-2 mediated by the arg-2 uORF and Arginine

M.S. / Molecular Biology / Neurospora crassa arg-2 encodes the small subunit of Arg-specific carbamoyl phosphate synthetase and is negatively regulated by arginine. This regulation is mediated by a 24-codon upstream open reading frame (uORF). The sequence of this uORF is critical for Arg-specific regulation. Six mutated templates were used to examine which residues of the uORF are important for this regulation. Mutations were created using megaprimer PCR and a luciferase gene was used as a reporter in the in vitro translation studies. Mutations of Asp 12, Asp 16, and Ser 10 eliminate Arg-specific regulation. Leaky scanning is thought to be involved, and a hypothetical ribosome stalling model that mediates Arg-specific attenuation of translation is proposed.

Identiferoai:union.ndltd.org:OREGON/oai:content.ohsu.edu:etd/188
Date06 1900
CreatorsCarroll, Julie Marie
PublisherOregon Health & Science University
Source SetsOregon Health and Science Univ. Library
LanguageEnglish
Detected LanguageEnglish
TypeText
FormatNeeds Adobe Acrobat Reader to view., pdf, 987.203 KB
Rightshttp://www.ohsu.edu/library/etd_rights.shtml

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