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The high performance liquid chromatography and detection of phospholipids and triglycerides /

Work on developing an instrumental system for total analysis of triglycerides and phospholipids is presented. Initial chromatographic analysis using reversed-phase high performance liquid chromatography on C(,6)-bonded, 5 (mu)m silica stationary phase and acetonitrile-water (and dilute phosphoric acid with phospholipids) mobile phase is demonstrated. The Kovats index is shown to allow correlation of structure to retention behavior. An ultraviolet absorbance detector ((lamda)(,195m,)) and novel post-column reaction detector (p.c.r.) based on saponification, periodate oxidation and derivatization using a new class of reagents are used for detection. The p.c.r. is shown to be sensitive to samples in the lower nanomole-per-injection range. / A novel transport-thermal ionic detector for organophosphorus compounds and a new class of aldehyde reagents are introduced. The detector is capable of detecting some pesticides in the lower nanogram-per-injection range. The aldehyde reagents, called the Fluorals, of which Fluoral-P (4-amino-3-penten-2-one) is studied extensively, have promise of being useful for selective, rapid and sensitive (sub-nanomoles formaldehyde) determinations.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68553
Date January 1980
CreatorsCompton, Bruce Jon.
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Chemistry)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 000127727, proquestno: AAINK51915, Theses scanned by UMI/ProQuest.

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