Immunoglobulin E (IgE)-conjugated gold nanoparticles were produced via in situ conjugation of gold nanoparticles with immunoglobulin E by laser ablation of Au in a liquid solution. The colloidal stability and the size distribution of the resulting bio-nanoconjugates were examined with UV-Visible spectroscopy (UV-Vis), dynamic light scattering (DLS), and transmission electron microscopy (TEM). These techniques showed that the Au nanoparticles in aqueous solutions were highly monodispersed spherical particles with a very narrow size distribution. The particles net diameter using TEM, was found to be D5 =3.8±0.9nm and D10 =4.7±1.3nm while the hydrodynamic diameter obtained with DLS was found to be h5 D =171±12nm , h10 D =164±18nm for 5min and 10min laser ablation time respectively. Enzyme-Linked immunosorbent Assay (ELISA) and flow cytometry measurements of the conjugates confirmed that the gold-bound protein remained biologically active, thus paving the way for the application of these nanoparticles in immuno-diagnostics, particularly in tumor-targeted drug delivery.
| Identifer | oai:union.ndltd.org:vcu.edu/oai:scholarscompass.vcu.edu:etd-1230 |
| Date | 12 May 2011 |
| Creators | MUTISYA, STEPHEN |
| Publisher | VCU Scholars Compass |
| Source Sets | Virginia Commonwealth University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
| Rights | © The Author |
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