For many years, the international scientific community has invested enormous efforts in the Human Genome sequencing project. The sequencing of the 23 human chromosomes is almost completed. This demonstrates that we now have to aim our efforts at improving their efficiency, and therefore resolution and speed are now the key issues. First, the effect of attractive analyte-gel interactions (we will study globular analytes here, proteins for example) will be examined within the framework of the Guo-Mercier-Slater model. It will be shown that it is possible to take into account such interactions and still calculate exact mobilities for various analytes and gel structures. Next, we will concentrate on the problems associated with the separation of chromosomal DNA (very long chains). Using Monte Carlo simulations, we will try to reproduce the dynamics of long DNA chains in three different experimental systems. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/9342 |
| Date | January 2000 |
| Creators | Labrie, Josée. |
| Contributors | Slater, Gary, |
| Publisher | University of Ottawa (Canada) |
| Source Sets | Université d’Ottawa |
| Detected Language | English |
| Type | Thesis |
| Format | 91 p. |
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