Phakopsora pachyrhizi, the causal agent of soybean rust (SBR), is a serious disease on soybeans.The objectives of this project were to identify additional alternative and possible overwintering hosts of the SBR pathogen and to validate the current detection assays for SBR. For the first objective, we attempted to identify naturalized Louisiana legume(s) that can serve as hosts and overwintering sites for P. pachyrhizi. It was theorized that New Iberia and Iberville Parishes in south Louisiana are usually the first locations of soybean infection in the spring. This suggests that there may be alternative leguminous hosts located in these parishes that may serve as overwintering hosts for P. pachyrhizi. Over 100 species of naturalized legumes were tested in field experiments to identify susceptible alternative hosts of the pathogen from November to May. For the second project, samples of P. pachyrhizi were collected from different parishes across Louisiana and sequenced across the ITS locus.These sequences were analyzed for variability within the locus and compared to the standard used in the current assay for pathogen detection. The Frederick et al. (2002) assay discriminates between P. pachyrhizi and P. meibomiae, a closely related mildly virulent species on soybeans in the US. A second assay, Barnes et al. (2009), was designed to detect a single spore of P. pachyrhizi in rain wash material, to monitor spore deposition and predict the movement of SBR in major soybean-producing states. However, the Barnes et al. (2009) assay has been shown to produce false positive and false negative results when used to detect the presence of P. pachyrhizi in North America. We tested five other Phakopsora spp. by both assays and found that only the Barnes et al. (2009) assay was able to detect all other Phakopsora spp. that were tested. Furthermore, the DNA segments used in the Barnes et al. (2009) assay, the primers had base pairs similarities that were 100% with other Phakopsora spp. and more than 60% with the specific P. pachyrhizi probe. In addition, we had determine that neither assay was placed in variable regions of the ITS locus.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-04192012-180014 |
| Date | 23 April 2012 |
| Creators | Rush, Tomas Allen |
| Contributors | Aime, M. Catherine, Schneider, Raymond W., Urbatsch, Lowell |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-04192012-180014/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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