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Adeno-associated virus rep78 expression in Arabidopsis thaliana

Adeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line. / Master of Science

Identiferoai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/9706
Date24 September 2002
CreatorsSisco, Daniel
ContributorsBiology, Lederman, Muriel L., Winkel, Brenda S. J., Rutherford, Charles L.
PublisherVirginia Tech
Source SetsVirginia Tech Theses and Dissertation
Detected LanguageEnglish
TypeThesis
FormatETD, application/pdf, application/pdf, application/pdf, application/pdf, application/pdf, application/pdf, application/pdf
RightsIn Copyright, http://rightsstatements.org/vocab/InC/1.0/
Relation03tableofcontents.pdf, 07references.pdf, 08resume.pdf, 04intro.pdf, 06resultsanddiscussion.pdf, 05materialsandmethods.pdf, 01title.pdf, 02abstract.pdf

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