A method for Agrobacterium-mediated transformation of hybrid
poplar (Populus alba x P. grandidentata) suspension cultures and
regeneration of transformed plants is described. The best protocol
was one in which suspension cultures were inoculated with
Agrobacterium tumefaciens to a density of 10⁷ cfu's/ml, cocultivated
for 48 hours, plated to cellulose acetate filters at a density of 14
colonies/mm², and cultured on medium containing 1 mg/1 2,4-D.
Although cefotaxime inhibited callus growth, it was used in the
plating medium to suppress proliferation of Agrobacterium. Selection
appeared to be more reliable using hygromycin as compared to
kanamycin or geneticin (G418). Transgenic plants were regenerated by
culturing the calli on media containing thidiazuron, but no shoots
could be regenerated using BA. / Graduation date: 1992
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/36532 |
| Date | 18 June 1991 |
| Creators | Howe, Glenn Thomas |
| Contributors | Strauss, Steve |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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