The production of barley (Hordeum vulgare L.) haploids by crossing with
H. bulbosum is a widely used tool in breeding and genetics. Certain barley
genotypes have low seed set in this interspecific cross, a phenomenon known
as partial incompatibility. Haploid production efficiency and gamete sampling
are important issues with the bulbosum technique, particularly when partially
incompatible genotypes are used. An in vitro floret culture system was
developed that substantially increases haploid production efficiency by
optimizing caryopsis growth, haploid embryo development, and plant
regeneration. The individual and combined effects of three plant growth
regulators (2,4-D, GA₃ and kinetin) on haploid production efficiency and its
determinants were compared in the floret culture system. 2,4-D alone was
superior to GA₃ alone in haploid production efficiency. 2,4-D alone or kinetin +
2,4-D are recommended for the purpose of haploid production in floret culture
using the bulbosum method.
Partial incompatibility between H. vulgare and H. bulbosum was studied
by doubled haploid progeny analysis. Two different loci were hypothesized to
account for the inheritance of partial incompatibility in the crosses of Vada x
Klages, Harrington x Klages, and Vada x Harrington. The partial incompatibility
gene in Harrington was found to be recessive. The dominant nature of the
partial incompatibility gene (Inc) in Vada was confirmed. An association
between the (Inc) gene and a deficiency in a stigma/stylodium-specific high pl
protein was found in the cosegregation analysis of doubled haploid progeny.
The Inc gene may be linked to the gene coding for the stigma/stylodium-specific
protein, or the Inc gene may regulate expression of the protein-encoding
locus. Segregation analysis of Mendelian markers in doubled haploid
progeny showed that there is no evidence that the partial incompatibility status
of the parents has an effect on gamete sampling by the bulbosum technique. / Graduation date: 1991
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/37323 |
| Date | 29 March 1991 |
| Creators | Chen, Fu-chiang, Chen, Fuqiang |
| Contributors | Hayes, Patrick M. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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