M.Sc. / Lotononis bainesii Baker and Lotononis listii Polhill both belong to the subsection Listieae (Fabaceae). Lotononis bainesii is utilised as a forage legume in certain parts of the world, but other species in the genus may also have potential use as forage. The influence of medium composition and pH on multiplication and rooting, as well as hardening and Rhizobium nodulation, was investigated for both species. Shoots were induced in L. bainesii on modified MS media (De Fossard, 1976), supplemented with 0.025 to lOmmoll i NAA and IAA, 0.025 to 0.5/Arno& BAP and Kinetin, 2% fructose, 0.6% agar, with the pH adjusted to 5.8. L. listii was regenerated on the same basic media, but containing 2% sucrose, 0.4% Gelrite and pH adjusted to 5.4. Rooting of L. listii were accomplished on modified MS medium supplemented with 0.5Amol.1-1 IBA and IAA, and 0.025/anol.14 BAP and Kinetin. Rooting and nodule formation under natural conditions were successful for L. listii, but less successful for L. bainesii. Possible differences between plants cultured in vitro and under natural conditions, were also examined using scanning electron- and light microscopy.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uj/uj:10016 |
| Date | 11 September 2012 |
| Creators | O'Brien, Oloff Marie |
| Source Sets | South African National ETD Portal |
| Detected Language | English |
| Type | Thesis |
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