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Transcript analysis of Feldmannia Sp. virus, FsV : characterization of the major capsid protein gene and its relationship to known viruses

The Feldmannia sp. virus is a large icosahedral virus that persistently infects
marine brown alga Feldmannia sp.. So far, there is no information available about
viral genome replication, gene structure and gene expression in this unique viral-host
system. The purpose of this study was to characterize the general features of viral
transcripts in the virus producing sporophyte plants. Northern analysis, using four
cosmid clones that cover the entire viral genome, showed that there were six major
transcripts and at least eighteen minor transcripts in the virus producing sporophyte
plants. These transcripts are not evenly distributed in the viral genome. A 5.7 kb
BamHI fragment-R was found to encode a 1.5 kb and a 0.9 kb major transcript, and
those two major transcripts were chosen for detailed sequence analysis. The 1.5 kb
transcript was identified as the putative major capsid protein (MCP) gene. The FsV
MCP has significant similarity with the major capsid protein of Chlorella virus-PBCV-1 and with iridoviruses, fish lymphocystis disease virus, frog virus 3, and with African
swine fever virus. / Graduation date: 1996

Identiferoai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/34633
Date26 April 1996
CreatorsJia, Yibing
ContributorsMeints, Russel H.
Source SetsOregon State University
Languageen_US
Detected LanguageEnglish
TypeThesis/Dissertation

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