Auxin controls a range of growth related characteristics by a mechanism
dependent upon polar auxin transport. We have identified a leaf vein patterning mutant
that shows a simple first leaf vein pattern. The veins are often non-meeting and form
somewhat parallel to one another. The leaves are narrow and pointed so that the overall
leaf phenotype is reminiscent of grass leaves; hence the mutant name grassy (gsy). A
range of shoot and root characteristics are also altered in gsy plants. Compared to wild
type, gsy plants have shorter primary roots with reduced numbers of lateral roots and
increased numbers of longer root hairs. Upon gravitropic stimulation, the root tip bends
slightly away from the normal vector. As well, gsy plants produce an inflorescence with
altered internode elongation and branching pattern. The intensity of the auxin responsive
reporter gene DR5::GUS is unchanged in both roots and developing leaves of gsy,
however, it shows subtle differences to the wild type DR5:GUS expression pattern.
Finally, gsy leaf and root phenotypes are more sensitive to low doses of the auxin efflux
inhibitor NPA and external auxin 2, 4-D. We suggest that this overall pattern is
consistent with defects in auxin transport. / xii, 76 leaves : ill. ; 29 cm.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:ALU.w.uleth.ca/dspace#10133/670 |
| Date | January 2008 |
| Creators | Pahari, Shankar, University of Lethbridge. Faculty of Arts and Science |
| Contributors | Schultz, Elizabeth |
| Publisher | Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2008, Arts and Science, Department of Biological Sciences |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_US |
| Detected Language | English |
| Type | Thesis |
| Relation | Thesis (University of Lethbridge. Faculty of Arts and Science) |
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