Protocols for the successful micropropagation of 'Queen Elizabeth' ('Q.E.') and 'Dick Koster' ('D.K.') roses were established, yielding a seven-ten fold multiplication rate per month. The effects of mechanically induced stress (MIS) (shaking stress) were evaluated on early establishment of greenhouse-grown 'Q.E.' and 'D.K.' rose cuttings and the ex vitro survival and hardiness of micropropagated 'Q.E.' plantlets. Shaking 'Q.E.' rose cuttings at 200 rpm for 30 min daily for 4 weeks during the rooting stage increased root length, dry weight and the root:shoot dry weight ratio. Similar shaking of 'D.K.' rose at 200 rpm for 15 min increased shoot fresh and dry weight and root length and dry weight. Prior to ex vitro acclimatization, plantlets shaken at 150 rpm for 15 min had reduced leaf dry weights. Those shaken at 200 rpm for 15 min had lower specific root water content but greater percent root dry matter. MIS was not directly implicated in improving ex vitro survival and hardiness of 'Q.E.' rose. (Abstract shortened by UMI.)
Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.61238 |
Date | January 1989 |
Creators | Korban, Martine |
Publisher | McGill University |
Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
Language | English |
Detected Language | English |
Type | Electronic Thesis or Dissertation |
Format | application/pdf |
Coverage | Master of Science (Department of Plant Science.) |
Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
Relation | alephsysno: 001069997, proquestno: AAIMM75842, Theses scanned by UMI/ProQuest. |
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