Jiang, Tiantian. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 61-75). / Abstracts in English and Chinese. / Thesis/Assessment Committee --- p.ii / Statement --- p.iii / Abstract --- p.iv / 摘要 --- p.v / Acknowledgements --- p.vi / List of Figures and Tables --- p.vii / Chapter Part 1 --- Introduction --- p.-0- / Chapter 1.1 --- Brassinosteriods (BRs) and BR signaling --- p.-0- / Chapter 1.1.1 --- Discovery of BRs --- p.-2- / Chapter 1.1.2 --- Functions of BRs --- p.-4- / Chapter 1.1.3 --- BR signaling pathway --- p.-6- / Chapter 1.2 --- Nitrogen (N) and N responses --- p.-10- / Chapter 1.2.1 --- Hormones involved in plant N responses --- p.-11- / Chapter 1.3 --- Anthocyanin and anthocyanin synthesis --- p.-13- / Chapter 1.3.1 --- Anthocyanin structures --- p.-13- / Chapter 1.3.2 --- Functions of anthocyanins --- p.-14- / Chapter 1.3.3 --- Biosynthesis of anthocyanins --- p.-14- / Chapter 1.3.4 --- Regulations of anthocyanin biosynthesis --- p.-15- / Chapter 1.4 --- Hormones and plant nutrient stresses --- p.-19- / Chapter Part 2 --- Materials and Methods --- p.-20- / Chapter 2.1 --- Plant materials and growth conditions --- p.-20- / Chapter 2.2 --- Measurement of anthocyanin content --- p.-21- / Chapter 2.3 --- Yeast two-hybrid (Y2H) assay --- p.-22- / Chapter 2.4 --- Bimolecular fluorescence complementation (BiFC) assays --- p.-23- / Chapter 2.5 --- Quantitative real-time PCR --- p.-25- / Chapter 2.6 --- Electrophoretic mobility shift assay (EMSA) and competition assay --- p.-26- / Chapter 2.7 --- Histochemical staining of GUS activity --- p.-28- / Chapter Part 3 --- Results --- p.-29- / Chapter 3.1 --- 24-epibrassinolide (24-eBR) increases plant tolerance to N-starvation in Arabidopsis - --- p.-29- / Chapter 3.2 --- BR treatment enhances anthocyanin accumulation under N deprivation conditions --- p.-31- / Chapter 3.3 --- BZR1 interacts with PAP1 in vitro and in vivo --- p.-35- / Chapter 3.4 --- BR and BZR1 promote the expression of the 'late' anthocyanin biosynthetic genes during N deprivation - --- p.-39- / Chapter 3.5 --- BZR1 binds to the promoter of DFR --- p.-43- / Chapter 3.6 --- BR-enhanced anthocyanin accumulation is specific to N-deprivation --- p.-46- / Chapter 3.7 --- BZR1 differently regulates PAP1 and PAP2 --- p.-48- / Chapter 3.8 --- Endogenous GL3 is required for BR-enhanced anthocyanin biosynthesis --- p.-52- / Chapter 3.9 --- N status affects the expression of BR biosynthetic gene CPD --- p.-52- / Chapter Part 4 --- Discussion --- p.-54- / Chapter 4.1 --- BRs confer plant tolerance to low-N stress and the tolerance is mediated by BR enhancement of low-N-induced anthocyanin biosynthesis --- p.-54- / Chapter 4.2 --- BRs enhance anthocyanin accumulation under N starvation through BZR1-PAP1 interaction or direct control of the expression of anthocyanin biosynthetic genes --- p.-55- / Chapter 4.3 --- BRs are specifically involved in low-N induced anthocyanin production --- p.-56- / Chapter 4.4 --- Transcription factors that specifically control BR-regulated anthocyanin biosynthesis --- p.-57- / Chapter 4.5 --- DFR is an important target of BR-regulation of anthocyanin biosynthesis --- p.-58- / Chapter Part 5: --- Conculsions --- p.-59- / Chapter Part 6: --- References --- p.-61-
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327364 |
| Date | January 2011 |
| Contributors | Jiang, Tiantian., Chinese University of Hong Kong Graduate School. Division of Life Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ix, 75 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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