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A comparative study of the in vitro antiproliferative activity of the extracts from the different developmental stages of pleurotus tuber-regium.

Wong Sze Man. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 124-144). / Abstracts in English and Chinese. / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Cancer treatment and potential novel antitumor agents --- p.1 / Chapter 1.2 --- History of mushroom polysaccharides in medical uses --- p.1 / Chapter 1.3 --- Life cycle of mushroom --- p.3 / Chapter 1.4 --- Classification of antitumor mushroom polysaccharides --- p.5 / Chapter 1.4.1 --- (3-glucans --- p.5 / Chapter 1.4.2 --- Heteropolysaccharides --- p.7 / Chapter 1.4.3 --- Polysaccharide-protein complexes --- p.7 / Chapter 1.5 --- Structure-activity relationship of mushroom polysaccharides --- p.8 / Chapter 1.5.1 --- Lentinan as typical example --- p.9 / Chapter 1.5.2 --- Molecular weight --- p.10 / Chapter 1.5.3 --- Conformation --- p.10 / Chapter 1.5.4 --- Chemical modification --- p.11 / Chapter 1.5.5 --- Degree of branching --- p.13 / Chapter 1.6 --- Antitumor mushroom polysaccharides obtained from different developmental stages --- p.17 / Chapter 1.7 --- Mechanisms of in vitro antitumor activity of mushroom polysaccharides: cell cycle arrest and apoptotic induction --- p.20 / Chapter 1.7.1 --- Cell cycle regulation --- p.21 / Chapter 1.7.2 --- Induction of apoptosis --- p.24 / Chapter 1.8 --- The novel strategies for cancer treatment --- p.27 / Chapter 1.9 --- Literature Review on Pleurotus tuber-regium --- p.30 / Chapter 1.10 --- Objectives --- p.33 / Chapter Chapter 2 --- Materials and Methods --- p.35 / Chapter 2.1 --- Materials --- p.35 / Chapter 2.1.1 --- Assay kits --- p.35 / Chapter 2.1.2 --- Mushroom samples --- p.35 / Chapter 2.1.3 --- Cell lines and their subculture --- p.36 / Chapter 2.1.4 --- Antibodies --- p.37 / Chapter 2.2 --- Extraction of mushroom polysaccharides --- p.38 / Chapter 2.2.1 --- Hot-water extracts from mushroom fruiting body --- p.38 / Chapter 2.2.2 --- Hot-water extracts from mushroom mycelia --- p.38 / Chapter 2.2.3 --- Exo-polysaccharides from submerged fermentation medium --- p.39 / Chapter 2.3 --- Chemical and physio-chemical composition of PTR extracts --- p.41 / Chapter 2.3.1 --- Neutral monosaccharides --- p.41 / Chapter 2.3.1.1 --- Acid Depolymerization --- p.41 / Chapter 2.3.1.2 --- Neutral sugar derivatization --- p.42 / Chapter 2.3.1.3 --- Determination of neutral sugar composition by GC- --- p.43 / Chapter 2.3.2 --- Uronic acid (acidic monosaccharides) content --- p.45 / Chapter 2.3.3 --- Total carbohydrate content --- p.46 / Chapter 2.3.4 --- Protein content --- p.46 / Chapter 2.3.5 --- Molecular weight and the homogeneity --- p.47 / Chapter 2.4 --- In vitro growth inhibitory effects --- p.48 / Chapter 2.4.1 --- Trypan blue dye exclusion method --- p.48 / Chapter 2.4.2 --- "Colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay" --- p.49 / Chapter 2.5 --- In vitro cell proliferation assay --- p.50 / Chapter 2.6 --- Cell-cycle analysis --- p.51 / Chapter 2.7 --- Apoptotic determination --- p.52 / Chapter 2.8 --- Expression of proteins involved in apoptosis and cell-cycle --- p.52 / Chapter 2.8.1 --- Preparation of cell lysates --- p.53 / Chapter 2.8.2 --- Determination of protein concentrations --- p.53 / Chapter 2.8.3 --- Western blot --- p.54 / Chapter 2.9 --- Statistics --- p.57 / Chapter Chapter 3 --- Results and Discussion --- p.58 / Chapter 3.1 --- Yield of extract samples isolated from different developmental stages of PTR --- p.58 / Chapter 3.2 --- Chemical characteristics of hot-water extracts isolated from different stages of PTR --- p.60 / Chapter 3.2.1 --- The total carbohydrate and protein content of PTR extracts- --- p.60 / Chapter 3.2.2 --- The monosaccharide composition of PTR extracts --- p.62 / Chapter 3.3 --- Molecular weight distribution of PTR extracts --- p.64 / Chapter 3.4 --- Chemical characterization of PTR extracts --- p.69 / Chapter 3.5 --- Cytotoxic effect of PTR extracts on various cell line in vitro --- p.71 / Chapter 3.5.1 --- Effect of PTR extracts on HL-60 cell viability --- p.71 / Chapter 3.5.2 --- Effect of PTR extracts on K562 cell viability --- p.74 / Chapter 3.5.3 --- Effect of PTR extracts on MCF-7 cell proliferation --- p.76 / Chapter 3.5.4 --- Effect of PTR extracts on HepG2 cell proliferation --- p.76 / Chapter 3.5.5 --- Effect of PTR extracts on normal cell proliferation --- p.78 / Chapter 3.6 --- Effect of PTR extracts on the proliferation rate of various cell lines in vitro --- p.78 / Chapter 3.6.1 --- Effect of PTR extracts on HL-60 cell proliferation --- p.79 / Chapter 3.6.2 --- Effect of PTR extracts on K562 cell proliferation --- p.79 / Chapter 3.6.3 --- Effect of PTR extracts on MCF-7 cell proliferation --- p.80 / Chapter 3.6.4 --- Effect of PTR extracts on HepG2 cell proliferation --- p.80 / Chapter 3.6.5 --- Effect of PTR extracts on normal cell proliferation --- p.84 / Chapter 3.7 --- Summary of the cytotoxic and antiproliferative activities exhibited by PTR extracts --- p.84 / Chapter 3.8 --- Analysis of the effect of PTR extracts on the cell-cycle phases of HL-60 and K562 cells --- p.87 / Chapter 3.8.1 --- Effect of CEP on cell-cycle phases of HL-60 and K562 cells --- p.87 / Chapter 3.8.2 --- Effect of EDP on cell-cycle phases of HL-60 and K562 cells --- p.92 / Chapter 3.8.3 --- Effect of HWE1 on cell-cycle phases of HL-60 and K562 cells --- p.95 / Chapter 3.8.4 --- Effect of HWE2 on cell-cycle phases of HL-60 and K562 cells --- p.98 / Chapter 3.8.5 --- Effect of HWE3 on cell-cycle phases of HL-60 and K562 cells --- p.102 / Chapter 3.8.6 --- Summary --- p.105 / Chapter 3.9 --- The effect of PTR extracts on expression of cellular proteins involved in cell-cycle control and apoptotic pathway in HL-60 cells --- p.106 / Chapter 3.9.1 --- Expression of Bcl-2 and Bax proteins in HL-60 cells treated with PTR extracts --- p.106 / Chapter 3.9.2 --- Expression of cyclins and Cdks in HL-60 cells by PTR extracts --- p.115 / Chapter 3.9.3 --- The plausible antiproliferative mechanism(s) involved in PTR extracts on HL-60 cells --- p.117 / Chapter Chapter 4 --- Conclusions and Future works --- p.120 / Chapter 4.1 --- Conclusions --- p.120 / Chapter 4.2 --- Future works --- p.122 / References --- p.124 / Related Publications --- p.144

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_325731
Date January 2006
ContributorsWong, Sze Man., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xviii, 144 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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