Osteoarthritis (OA) is a disease characterized by the progressive degradation of
articular cartilage. Current strategies for the disease are mainly towards
relieving symptoms. This study was aimed to investigate the therapeutic
potentials of Bone Morphogenetic Protein-9 (BMP-9), Raloxifene (Ral) and
Pluronic F-68 (PLF-68) with a three-dimensional in vitro OA model.
Articular chondrocytes isolated from rats were cultured in growth media and
embedded in agarose to obtain agarose-chondrocyte discs. Dynamic
hydrostatic mechanical stress was applied to discs. The discs were incubated
with Aza-C for 48 hours for OA development. After its removal, chondrocytes
were treated with different doses of BMP-9, Ral and PLF-68 for 10 days. The
efficacies of treatments were evaluated by measuring cell number,
glycosaminoglycan and collagen amount, and mechanical properties of the
v
discs. Measurements of these properties were performed with MTT,
quantitative colorimetric assays, histochemical staining and mechanical tests,
respectively.
According to comparative results with healthy groups and controls
(osteoarthritic chondrocytes without any treatment), it was found that BMP-9
had negative effect on osteoarthritic chondrocytes. On the other hand, Ral
showed positive results related with matrix synthesis and mechanical properties
especially at 5 & / #956 / M dose suggesting that it holds promise for the treatment of
OA. The therapeutic effect of Ral on OA was documented for the first time in
literature. The potential of PLF-68 for treatment of OA was also supported by
this study considering its positive effects on cell number, collagen synthesis
and mechanical properties. Yet, further investigations are also suggested for
conclusive results on this agent.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12608800/index.pdf |
| Date | 01 September 2007 |
| Creators | Kavas, Aysegul |
| Contributors | Tezcaner, Aysen |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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