Polychlorinated biphenyls (PCBs) are chlorinated organic compounds, which belong to persistent organic pollutants and exhibit various modes of toxic action, including mutagenicity, carcinogenicity, and endocrine disruption. PCBs were manufactured during the 20th century in many countries and extensively used due to their advantageous physicochemical properties. PCBs mostly served as insulating liquids in electrical equipment; however, they were also utilized in many open applications. Despite the worldwide ban on PCB manufacture imposed at the end of the 20th century, the contamination of the environment persists to this day as a result of their recalcitrance. Moreover, PCBs are still being inadvertently produced during many industrial activities. Because of their stability, the breakdown of PCBs in nature is extremely slow. This dissertation thesis focuses on the study of PCB biodegradation by ligninolytic fungi. This group of microorganisms belongs to the most promising, especially in regard to the degradation of organic pollutants. The biodegradation mechanism of PCBs was studied in vitro, including the identification of degradation intermediates. Laccase, an enzyme expressed by the oyster mushroom (strain Pleurotus ostreatus 3004), was able to degrade hydroxylated PCBs. In addition, chlorinated...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:453329 |
| Date | January 2021 |
| Creators | Šrédlová, Kamila |
| Contributors | Cajthaml, Tomáš, Kočí, Vladimír, Macek, Tomáš |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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