The objective of this dissertation was to develop novel methods of patterning inorganic and organic materials, develop biocompatibility evaluations, and subsequently apply these methods toward developing biosensors and lab-on-a-chip devices, such as Interdigitated Electrodes (IDEs) and Microelectrode Arrays (MEAs) on non-traditional (such as nanostructured and plasmonic) polymer substrates or deploy these methods to enhance precision cellular placement on traditional (glass) MEA substrates. It was hypothesized that a combination of such facile microfabrication techniques and patterning technologies on traditional and non-traditional substrates would increase the sensitivity and selectivity of such sensor platforms by several orders of magnitude, and potentially introduce new modalities for cell-based biosensing. In order to demonstrate the biological functionality of these new IDEs and MEAs, a variety of cell cultures were used (cardiac, stem cell, and endothelial cells) to study the growth, proliferation, modes of increasing sensitivity and response to various compounds in vitro (outside the body).
| Identifer | oai:union.ndltd.org:ucf.edu/oai:stars.library.ucf.edu:etd2020-1800 |
| Date | 01 January 2020 |
| Creators | Hart, Cacie |
| Publisher | STARS |
| Source Sets | University of Central Florida |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Electronic Theses and Dissertations, 2020- |
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