Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Propolis is a resinous material collected by bees from different parts of plants. Both its composition as its biological properties are dependent on factors such as climate, soil, vegetation and species of collecting bees. Propolis has great potential for therapeutic use due to its chemical composition and pharmacological properties. Herein it was determined the contents of phenolic compounds, antioxidant capacity and chemical composition (assessed by HPLC-PAD) of twelve green propolis samples from beekeepers and sixteen commercial propolis extracs from different regions of Southeast Brazil. The antioxidant abilities of the extracts were qualitatively determined through the total phenolic contents using the Folin?Ciocalteau method. Total flavonoids were assessed by the method of complexation with aluminium chloride. The quantitative antioxidant activiti of propolis extracts were determined both by trapping the organic radicals DPPH and ABTS.+ as well by the iron reduction method (FRAP). High pressure liquid chromatography allowed the identification and quantification of chlorogenic acid, caffeic acid, ferulic acid, para-coumaric acid, rosmarinic acid, vanillin, hesperidin, naringenin, pinobanksin, kaempferol, 4-hydroxy-3,5-diprenyl cinnamic acid (Artepillin-C), kampheride and pinostrobin. Artepillin C, a well known chemical marker of propolis, was present in all propolis extracts analyzed. We highlight that the presence of rosmarinic acid in propolis samples from Rio de Janeiro was reported for the first time in this work. The discrimination between green propolis in natura and commercial green propolis extracts was performed using PCA (Principal Component Analysis) statistical method. The antioxidant capacity in vivo of propolis extracts were evaluated using Saccharomyces cerevisiae as biological system model, assessing important parameters as stress tolerance and lipid peroxidation rate. The antifungal activity of ethanol extracts of propolis in natura were tested against the phytopathogenic fungus Fusarium oxysporium. / A pr?polis ? um material resinoso coletado pelas abelhas de diferentes partes das plantas, sua composi??o e as suas propriedades biol?gicas dependem do clima, solo, vegeta??o e da esp?cie da abelha. A pr?polis tem grande potencial de aplica??o terap?utica, devido ? sua composi??o e propriedades farmacol?gicas. Nesse trabalho foi determinado o teor de subst?ncias fen?licas, a capacidade antioxidante e composi??o qu?mica por CLAE-DAD de doze amostras de pr?polis verde, adquiridas de apicultores, e dezesseis extratos de pr?polis comerciais, provenientes de diferentes regi?es do Sudeste do Brasil. A capacidade antioxidante dos extratos foi avaliada qualitativamente atrav?s do teor de fen?licos totais, pelo m?todo de Folin?Ciocalteau, e flavonoides pelo m?todo de complexa??o com cloreto de alum?nio. A quantifica??o do potencial antioxidante foi realizada pela captura dos radicais org?nicos DPPH e ABTS.+, al?m do m?todo de redu??o do ?on f?rrico (FRAP). An?lises por cromatografia l?quida de alta efici?ncia permitiram a identifica??o e quantifica??o de ?cido clorog?nico, ?cido cafeico, ?cido fer?lico, ?cido para-cum?rico, ?cido rosmar?nico, ?cido 3,5-diprenil-4-hidroxicin?mico (Artepillin C), vanilina, hesperidina, naringenina, pinobanksina, canferol, canferide e pinostrobina. Em todos os extratos de pr?polis analisados foi identificado o Artepillin C, marcador qu?mico para pr?polis verde. Destacamos que neste trabalho foi reportada pela primeira vez a presen?a de ?cido rosmar?nico em pr?polis do Rio de Janeiro. M?todos quimiom?tricos de an?lise explorat?ria, utilizando ?nalise por Componentes Principais foram utilizados para discriminar extratos de pr?polis verde in natura daqueles extratos comerciais. O potencial antioxidante in vivo dos extratos etan?licos de pr?polis foram avaliados utilizando cepas controles de Saccharomyces cerevisiae como modelo de sistema biol?gico e foram avaliados toler?ncia ao estresse e proxida??o lip?dica. A atividade antif?ngica dos extratos etan?licos de pr?polis in natura foram avaliados, e inibiram o crescimento in vitro do fungo fitopat?geno Fusarium oxysporum.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1469 |
| Date | 23 September 2016 |
| Creators | SALGUEIRO, Fernanda Barbosa |
| Contributors | Castro, Rosane Nora, Valverde, Alessandra Leda, Moreira, Davyson de Lima, Lorenzon, Maria Cristina Affonso, Suzart, Luciano Ramos |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Qu?mica, UFRRJ, Brasil, Instituto de Ci?ncias Exatas |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Unknown |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7 - REFERENCIAS BIBIOGR?FICAS ADAMIS, P.D.B.; GOMES, D.S.; PEREIRA, M.D.; MESQUITA, J.F.; PINTO, M.L.C.C.; PANEK, A.D.; ELEUTHERIO, E.C.A. The effect of superoxide dismutase deficiency on cadmium stress. Journal of Biochemical and Molecular Toxicology.18, 12?17, 2004 AGA,H.; SHIBUYA,T.; SUGIMOTO,T.; KURIMOTO,M.; NAKAJlMA,S. Isolation and Identification of Antimicrobial Compounds in Brazilian Pr?polis. Bioscience, Biotechnology and Biochemistry. 58, 945-946, 1994. AGRAWAL, P. K. Carbon-13 NMR of flavonoids. Elsevier: Amsterdam ? Oxford ? New York ? Tokyo, 564 p. 1989. ALENCAR, S. M.; OLDONI, T. L. C.; CASTRO, M. L.; CABRAL, I. S. R.; COSTA-NETO, C. M.; CURY, J. A.; ROSALEN, P. L.; IKEGAKI, M. Chemical composition and biological activity of a new type of Brazilian propolis: Red propolis. Journal of Ethnopharmacology. 113, 278-283, 2007. ALENCAR, S.M.; AGUIAR, C.L.; GUZM?N, J.P.; PARK,Y.K. Composi??o qu?mica de Baccharis dracunculifolia. Ci?ncia Rural. 35: 909-915, 2005. ALVES, C.Q.; DAVID, J.M.; DAVID, J.P.; BAHIA, M.V.; AGUIAR, R.M. M?todos para determina??o de atividade antioxidante in vitro em substratos org?nicos. Qu?mica Nova. 33, 2202-2210, 2010 ALVES, E.; KUBOTA, E.H. Conte?do de fen?licos, flavonoides totais e atividade antioxidante de amostras de pr?polis comerciais. Revista de Ci?ncias Farmac?uticas B?sica e Aplicada. 34, 37-41, 2013. ALVES, E.J.; SHEPHERD, K.; DANTES, J.L.L. Cultivation of bananas and plantains in Brazil and needs for improvemeny. Banana and Plantain Breeding Strategies. Aciar Proceegings 21. Australia, 1986. BANKOVA, V.Chemical diversity of propolis and the problem of standardization. The Journal of Ethnopharmacology .100, 114-117, 2005. BANKOVA, V. BOUDOUROVA-KRASTEVA, G.; SFORCIN, J.M.; FRETE, X.; KUJUMGIEV, A.; MAIMONI-RODELLA, R.; POPOV, SPhytochemical Evidence for the Plant Origin of Brazilian of Brazilian Propolis from S?o Paulo. Zeitschirift fur Naturforschung, 54, 402-405, 1999. BANKOVA, V.; CASTRO, S.L.D.; MARCUCCI, M. Propolis: recent advances in chemistry and plant origin. Apidologie. 31, 3?15, 2000. BANKOVA, V.; CHRISTOV, R.;KUJUMGIEV, A.;MARCUCCI, M.C.;POPOV, S. Chemical composition and antibacterial activity of Brazilian propolis. Zeitschrift f?r Naturforschung C. 50, 167-172, 1995 146 BELFAR, M.L.; LANEZ, T.; REBIAI, A.; GHIABA, Z Evaluation of antioxidant capacity of propolis collected in various areas of Algeria using electrochemical techniques. International Journal of Electrochemical Science. 10, 9641 ? 9651, 2015. BENZIE, I.F., STRAIN, J. The ferric reducing ability of plasma (FRAP) as a measure of ??Antioxidant Power?: the FRAP assay. Analytical Biochemistry. 239, 70?76, 1996. BERTONCELJ, J., DOBERSEK, U., JAMNIK, M., GOLOB, T. Evaluantion of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chemistry. 105,822?828, 2007 BITTENCOURT, M.L.F.; RIBEIRO, P.R.R.; FRANCO, R.L.P.; HILHORST, H.W.M.; CASTRO, R.D.; FERNANDEZ, L.G. Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis: Use of correlation and multivariate analyses to identify potential bioactive compounds. http://dx.doi.org/10.1016/j.foodres.2015.07.008. Food Research International. 2015 BITTERMAN,K.J.;MEDVEDIK, O.; SINCLAIR, D.A.Longevity Regulation in Saccharomyces cerevisiae: Linking Metabolism, Genome Stability, and Heterochromatin. Microbiology and Molecular Biology Reviews.67, 376-399, 2003 BOFFO, E. F. Tese Doutorado. Utiliza??o de RMN aliada a M?todos Quimiom?tricos na an?lise de mel e aguardentes. S?o Carlos ?UFSCar, 2009. BOISARD, S.; LE RAY, A.; GATTO, J.; AUMOND, M.; BLANCHARD, P.; DERBR , S.; FLURIN, C.; RICHOMME, P. Chemical composition, antioxidant and anti-ages activities of a French poplar type pr?polis. Journal of Agricultural and Food Chemistry. 62, 1344-1351, 2014. BONVEHI, J.S.; COLL, F.V. Phenolic Composition of Propolis from China and from South America. Zeitschrift Fur Naturforschung C. 49, 712-718, 1994. BOUFADI, Y.M.; SOUBHYE, J.; RIAZI, A.; ROUSSEAU, A.; VANHAEVERBEEK, M.; N?VE, J.; BOUDJELTIA, K.Z.; ANTWERPEN, P.V. Characterization and antioxidant properties of six Algerian pr?polis extracts: ethyl acetate extracts inhibit myeloperoxidase activity. International Journal of Molecular Sciences. 15, 2328, 2014. BRAND-WILLIAMS, W.; CUVELIER, M.E.; BERSET, C. Use of a free radical method to evaluate antioxidant activity. Food Science and Technology. 28, 25-30, 1995. BRASIL. Instru??o Normativa n.? 3, de 19 de janeiro de 2001. Regulamentos T?cnicos de Identidade e Qualidade de Apitoxina, Cera de Abelha, Gel?ia Real, Gel?ia Real Liofilizada, P?len Ap?cola, Pr?polis e Extrato de Pr?polis. Minist?rio da Agricultura, Pecu?ria e Abastecimento, Bras?lia, DF. 23 de jan. 2011. Se??o 1, p. 18- 23. CABRAL, I S. R.; OLDONI, T. L. C.; PRADO, A.; BEZERRA, R. M. N.; ALENCAR, S. M. Composi??o fen?lica, atividade antibacteriana e antioxidante de pr?polis vermelha brasileira. Qu?mica Nova, 32, 1523 - 1527, 2009 CAETANO, F. M. Disserta??o de mestrado. Metodologias de extra??o de pr?polis do Sul de Minas Gerais. Universidade S?o Francisco. 2000. 147 CAETANO, S.C.M.O. Disserta??o de Mestrado. Yap1 no stress causado pelo excesso de cobalto em S. cerevisiae. Universidade de Aveiro. 2011. CAPISTRANO, H.M.; ASSIS,E.M.A.; R,M.; ALVAREZ-LEITE, M.E.; BRENER,S.; BASTOS, E.M.A.F. Brazilian green propolis compared to miconazole gel in the treatment of candida-associated denture stomatitis. Evidence-Based Complementary and Alternative Medicine. 947980, 6, 2013. CHANG, R.; PIL?-VELOSO, D.; MORAIS, S.A. L.; NASCIMENTO, E.A. Analysis of a Brazilian green propolis from Baccharis dracunculifolia by HPLC-APCI-MS and GC-MS. Brazilian Journal of Pharmacognosy. 18, 4, 549-556, 2008 CHEN, M.; WEI, H.; ZHANG, C.; FANG, Y. Review: High performance liquid chromatography for the determination of flavonoids. Journal of Chinese Pharmaceutical Sciences. 20; 313?324, 2011 COTTICA, S. M.; SAWAYA, A.C.H.F.; EBERLIN, M.N.; FRANCO, S.L.; ZEOULA, L.M.; VISENTAINERA, J.V. Antioxidant activity and composition of propolis obtained by different methods of extraction. Journal of the Brazilian Chemical Society, 22, 929-935, 2011 CUNHA, I.B.S.; SAWAYA, A. C. H. F.; CAETANO.; F.M.; SHIMIZUA, M.T.; MARCUCCI, M.C.; DREZZA, F.T.; POVIA, G.S.; CARVALHO, P.O. Factors that influence the yield and composition of brazilian propolis extracts. Journal of the Brazilian Chemical Society. 15, 964-970, 2004. CURIFUTA. M; VIDAL, J.; S?NCHEZ-VENEGAS, J.; CONTRERAS, A.; SALAZAR, L.A.; ALVEAR, M. The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance. Ci?ncia e Investiga??o Agr?cola. 39, 347?359, 2012. CUSTODIO,A.R.,FERREIRA, M.M. C.; GIUSEPPINA NEGRI, G.; SALATINO, A, Clustering of comb and propolis waxes based on the distribution of aliphatic constituents. Journal of the Brazilian Chemical Society, 14, 354-357, 2003 DA SILVA FILHO, A.A. Tese de Doutorado.Estudo fitoqu?mico, de cultivo e das atividades biol?gicas de Baccharis dracunculifolia D.C. (Asteraceae), a principal fonte bot?nica da pr?polis verde. Ribeir?o Preto-SP. Universidade de S?o Paulo.2006. DALBEN-DOTA, K.F.; FARIA, M.G.; BRUSCHI, M.L.; PELLOSO, S.M.;LOPES-CONSOLARO, M.E.; SVIDZINSKI, T.I. Antifungal activity of propolis extract against yeasts isolated from vaginal exudates. Journal of Alternative and Complementary Medicine. 16, 285-90, 2010. DALBEN-DOTA, K.F.; LOPES-CONSOLARO, M.E.; SVIDZINSKI, T.I.; BRUSCHI, M.L. Antifungal activity of brazilian propolis microparticles against yeasts isolated from Vulvovaginal Candidiasis. Evidence-Based Complementary and Alternative Medicine. 201953, 8, 2011. DALEPRANE, J.B.; ABDALLA D.S. Emerging Roles of Propolis: Antioxidant, Cardioprotective, and Antiangiogenic Actions. Evidence-Based Complementary and Alternative Medicine. 175135, 8, 2013. 148 DE-MELO, A.A.M.; MATSUDA, A.H.; FREITAS, A.S.; BARTH,O.M.; ALMEIDA-MURADIAN, L.B. Capacidade antioxidante da pr?polis. Pesquisa Agropecu?ria Tropical. 44, 341-348, 2014. DEWICK, P.M. Medicinal Natural Products: A Biosynthetic Approach. 2? ed. John Wiley & Sons, LTD, 2002 ERIN, S.M. Tese de doutorado. Contribuici?n al estabelecimiento de las bases cient?ficas para el uso de fracciones polifen?licas y fibra diet?tica antioxidante em la prevenci?n del c?ncer. Universidade de Barcelona, 2009. FABRIS, S.; BERTELLE, M.; ASTAFYEVA, O.; GREGORIS, E.; ZANGRANDO, R.; GAMBARO, A.; LIMA, G.P.L.; STEVANATO, R. Antioxidant properties and chemical composition relationship of europeans and brazilians propolis. Pharmacology & Pharmacy. 4, 46-5, 2013. FALC?O, S. I.; VALE, N.; GOMES, P.; DOMINGUES, M. R., FREIRE, C.; CARDOSO, S.M.; VILAS-BOA, M. Phenolic profiling of portuguese propolis by LC?MS spectrometry: uncommon propolis rich in flavonoid glycosides. Phytochemical Analysis. 24, 209-318, 2013. FALC?O, S.I.; TOM?S, T.; VALE, N.; GOMES, P.; FREIRE, C.; VILAS-BOAS, M. Phenolic quantification and botanical origin of Portuguese pr?polis. Industrial Crops and Products. 49, 805- 812, 2013. FERNANDES, A.; BALESTRIN, E.C.; BETONI, J.E.C.; ORSI, R.O.; CUNHA, M.L.R.S.; MONTELLI, A.C. Propolis: anti- Staphylococcus aureus activity and synergism with antimicrobial drugs. Mem?rias do Instituto Oswaldo Cruz 100, 563-66, 2005. FERNANDES-SILVA, C.C.; SALATINO, A.; SALATINO, M.L.F.; BREYER, E.D.H.; NEGRI, G. Chemical profiling of six samples of brazilian propolis. Qu?mica Nova. 36, 237-240, 2013 FERREIRA, T. C. Tese de Doutorado. Clonagem e express?o do fator 1 humano induz?vel por hip?xia (HIF-1) na levedura Saccharomyces cerevisiae. Universidade de Bras?lia, 2006. FIGUEIREDO, S.M.F.; BINDA, N.S.; ALMEIDA, B. M.; ABREU, S.R.L.; ABREU, J.A.S.; PASTORE, G.M.; SATO, H.H.; TORETI, V.C.; TAPIA, E.V.; PARK, Y.K.; VIEIRA FILHO, S.A.; CALIGIORNE, R.B. Green Propolis: Thirteen Constituents of Polar Extract and Total Flavonoids Evaluated During Six Years through RP-HPLC. Current Drug Discovery Technologies. 12, 00-001, 2015. FONTANA, J. D., ADELMANN, J., Passos, M., Maraschin, M., Lacerda, C. A. de e Lan?as, F. M. Propolis: chemical micro-heterogeneity and bioactivity. In: New Jersey: Humana press, 2004. p. 203-218. FREIRES, I.A.; QUEIROZ, V.C.; FURLETTI, V.F.; IKEGAKI, M.; ALENCAR, S.M.; DUARTE, M.C.; ROSALEN, P.L. Chemical composition and antifungal potential of Brazilian propolis against Candida spp. Journal of Medical Mycology. 26, 122-132, 2016. 149 FREITAS, A.S.; BARTH, O.M. & LUZ, C.F.P. Pr?polis marrom da vertente atl?ntica do Estado do Rio de Janeiro, Brasil: uma avalia??o palinol?gica. Revista Brasileira de Bot?nica, 33,343-354, 2010. GARDANA, C., SCAGLIANTI, M., PIETTA, P., SIMONETTI, P. Analysis of the polyphenolic fraction of pr?polis from different sources by liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 45, 390-399, 2007. GHISALBERTI, E. L. Propolis: a review. Bee World,.60, 59-84, 1979. GONG, S.; LUO, L.; GONG, W.; GAO, Y.; XIE, M. Multivariate analyses of element concentrations revealed the groupings of propolis from different regions in China. Food Chemistry.134, 583-588, 2012. GRAIKOU, K. ; POPOVA, M.; GORTZI, O.; BANKOVA, V.; CHINOU,I. Characterization and biological evaluation of selected Mediterranean propolis samples. Is it a new type? LWT - Food Science and Technology, 65, 261-267, 2016. GREENAWAY, W., SCAYSBROOK, T., WHATLEY, F.R. The composition and plant origins of propolis: a report of work at Oxford. Bee World. 71,107-118, 1990. GREG?RIO, L.E. Disserta??o de Mestrado. Influ?ncia da sazonalidade na composi??o pol?nica, no perfil qu?mico e na atividade antimicrobiana da pr?polis produzida em Cajur?-SP. Ribeir?o Preto-SP. Universidade de S?o Paulo. 2003. GREGORIS, E.; STEVANATO, R. Correlations between polyphenolic composition and antioxidant activity of Venetian propolis. Food Chemistry, 48, 1, 76-82, 2010 GUARIENTI, C.; BERTOLIN, T. E. COSTA, J. A. V. Capacidade antioxidante da microalga Spirulina platensis em c?lulas da levedura Saccharomyces cerevisiae submetidas ao estressor paraquat. Revista Instituto Adolfo Lutz. 69, 106-111, 2010. GUL?IN, I. Antioxidant activity of food constituents: an overview. Archives of Toxicology. 86, 345?391, 2012. HASHEM, A.; ABD-ALLAH, E.F.; ALWATHNANI, H.A. Effect of propolis on growth, aflatoxins production and lipid metabolism in aspergillus parasiticus spear. Pakistan Journal of Botany. 44, 1153-1158, 2012. HALLIWELL, B.; AESCHBACH, R.; LOLIGER, J.; AROUMA, O. I.; The Characterization of Antioxidants. Food and Chemical Toxicology. 33, 601, 1995 HALLIWELL, B.; GUTTERIDGE, J. M. C.; Free Radical in Biology and Medicine, 3rd ed., Oxford University Press: Oxford, 4th ed. 2002 HAYASHI, K.; KOMURA, S.; ISAJI, N.; OHISHI, N.; YAGI, K. Pharmaceutical Society of Japan Isolation of Antioxidative Compounds from Brazilian Propolis: 3,4-Dihydroxy-5-prenylcinnamic Acid, a Novel Potent Antioxidant. Chemical and Pharmaceutical Bulletin. 47, 1521-1524, 1999. 150 HELENO, S.A.; MARTINS, A.; QUEIROZ, M.J.R.P.; FERREIRA, I.C.F.R. Bioactivity of phenolic acids: Metabolites versus parent compounds: A review. Food Chemistry. 173,501?513, 2015 HUANG , S., ZHANG, C., WANG, K., LI, G.Q., HU, F. Recent advances in the chemical composition of propolis. Molecules, 19, 19610-19632, 2014. JUG, M.; KONCIC, M.Z.; KOSALEC, I. Modulation of antioxidant, chelating and antimicrobial activity of poplar chemo-type propolis by extraction procures. LWT - Food Science and Technology. 57, 530-537, 2014. KALEMBA, D.; KUNICKA, A. Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry. 10, 813-29, 2003. KALIL, K. M.; DE VILLIERS, A. Review: Recent developments in the HPLC separation of phenolic compounds. Journal of Separation Science. 34:8, 854?876, 2011 KALINOWSKA, M.; LADERIERE, B.; CHAMPAGNE, P.; KOWCZYK-SADOWY, M.; LEWANDOWSKI, W. Mn(II), Cu(II) and Cd(II) p-coumarates: FT-IR, FT-Raman, 1H and 13C NMR and thermogravimetric studies. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 103, 264?271, 2013 KUJUMGIEV, A., TSVETKOVA, I., SERKEDJIEVA, Y., BANKOVA, V.S., CHRISTOV, R., POPOV, S. Antibacterial, antifungal and antiviral activity of pr?polis of different geographic origin. Journal of Ethnopharmacology, 64, 235?240, 1999. KUMAZAWA, S., YONEDA, M., SHIBATA, I., KANAEDA, J., HAMASAKA, T., NAKAYAMA, T. Direct evidence for the plant origin of Brazilian propolis by the observation of honeybee behavior and phytochemical analysis. Chemical and Pharmaceutical Bulletin. 51, 740-742, 2003. KUMAZAWA, S.; BONVEH?, J.S.; TORRES, C.; AHN MOK-RYEON, A.; BERMEJO F.J.O. Chemical and functional characterisation of propolis collected from East Andalusia. (Southern Spain). Phytochemical Analysis . 24, 608?615, 2013. KUREK-G?RECKA, A.; RZEPECKA-STOJKO, A.; G?RECKI, M.; STOJKO, J.; SOSADA, M.; ?WIERCZEK-ZI?BA.; G. Structure and antioxidant activity of polyphenols derived from propolis. Molecules, 19, 78-101, 2014. KUROPATNICKI, A.K.; SZLISZKA, E.; KROL, W. Historical Aspects of Propolis Research in Modern Times. Evidence-Based Complementary and Alternative Medice. 964149, 11, 2013. LIMA, M.G. A produ??o de pr?polis no Brasil. S?o Jo?o da Boa Vista: S?o Sebasti?o Editora e Gr?fica. 2006. LIMA, F.O.; BEZERRA, A.S. Disciplinarum Scientia. S?rie: Ci?ncias Naturais e Tecnol?gicas, Santa Maria, 13, 111-124, 2012. LIMA, M.M.O.; VIEIRA, L. F.; COSTA JUNIOR, J. S. Avalia??o da atividade antioxidante de Platonia Insignis Mart. (Clusiaceae). In: II Congresso de Pesquisa e Inova??o da Rede Norte Nordeste de Educa??o Tecnol?gica, Jo?o Pessoa, Para?ba, 2007. 151 LONGHINI, R.; RAKSA, S.M.; OLIVEIRA, A.C.O.; SVIDZINSKI, T.I.E.; FRANCO, S.L. Obten??o de extratos de pr?polis sob diferentes condi??es e avalia??o de sua atividade antif?ngica. Brazilian Journal of Pharmacognosy. 17, 388-395, 2007 LUSTOSA, S.R. GALINDO, A.B. NUNES, L, C.C. RANDAU, K.P. NETO, P.J.R. Pr?polis: atualiza??es sobre a qu?mica e a farmacologia. Revista Brasileira de Farmacognosia. 18, 447-454, 2008. MABRY, T.J; MARKHAM, K.R.; THOMAS, M.B. 1970: Systematic identification of flavonoids. Springer-Verlag. New York, USA MACHADO, P.P.; VIEIRA, G.H.C.; MACHADO, R.A. Uso da pr?polis e ?leo de nim no controle dos fungos Lasiodiplodia theobromae e Colletotrichum gloesporioides: principais pat?genos que acometem os frutos da manga. Revista de Agricultura Neotropical. 2, 31-37, 2015. MARCUCCI , M. C; CUST?DIO, ?. R. Pr?polis: Correla??o Qu?mica e Biol?gica. Chemkeys - Liberdade para aprender..1-23, 2002. MARCUCCI, M.C. Propolis: chemical composition, biological properties and therapeutic activity, Apidologie, 26, 83?99, 1995. MARCUCCI, M.C.; RODRIGUEZ, J.; FERRERES, F.; BANKOVA, V.; GROTO, R.; POPOV, S. Chemical Composition of Brazilian Propolis from Sao Paulo State. Zeitschrift f?r Naturforschung C. 53, 117-119, 1998 MARINI, D.; MENSCH, R.; FREIBERGER, M.B.; DARTORA, J.; FRANZENER, G.; GARCIA, R.C.; STANGARLIN, J.R. Efeito antif?ngico de extratos alco?licos de pr?polis sobre pat?genos da videira. Arquivos do Instituto Biol?gico. 79,.305-308, 2012. MARKHAM, K.R.., Techniques of flavonoid identification. 1982: Academic Press, London. MATSUDA, A. H.; ALMEIDA-MURADIAN, L. B. Validated method for the quantification of Artepillin C in Brazilian propolis. Phytochemical Analysis, 19, 2, 179-183, 2008 MELLO, B.C.B. S.; PETRUS, J.C.C. , HUBINGER,M.D. Desempenho do processo de concentra??o de extratos de pr?polis por nanofiltra??o. Ci?ncia e Tecnologia de Alimentos. 30, 166-172, 2010 MENESES, E.A.; DURANGO, D.L., GARC?A, C.M. Antifungal activity against postharvest fungi by extracts from Colombian propolis. Qu?mica Nova 32, 2011?2017, 2009. MENSOR, L.L.; MENEZES, F.S.; LEIT?O, G.G.; REIS, A.S.; DOS SANTOS, T.C.; COUBE, C.S.; LEIT?O, S.G. Screening of Brazilian plant extracts for antioxidant acitivity by the use of DPPH free radical method. Phytotherapy Research. 15,2, 127-130, 2001 MERABET, L.P. Determina??o da atividade de ?gua, teor de umidade e par?metros microbiol?gicos em compostos de mel. Revista Brasileira de Economia Dom?stica. 22, 213-232, 2011 152 MERKEN, H. M., BEECHER, G. R. Measurement of food flavonoids by high performance liquid chromatography: A review. Journal of Agricultural and Food Chemistry. 48, 577-599, 2000 MIGUEL, M.G.; NUNES, S.; DANDLEN, S.A.; CAVACO, A.M.; ANTUNES, MD.Phenols, flavonoids and antioxidant activity of aqueous and methanolic extracts of propolis (Apis mellifera L.) from Algarve, South Portugal. Food Science and Technology. 34, 16-23, 2014 MIHAI C.M.; M?RGHITA? L.A.; DEZMIREAN D.S.; CHIRIL? F.; MORITZ R.F.; SCHL?NS H. Interactions among flavonoids of pr?polis affect antibacterial activity against the honeybee pathogen Paenibacillus larvae. Journal of Invertebrate Pathology. 110, 68?72, 2012. MILLER, N. J., RICE-EVANS, C., DAVIES, M. J., COPINATHAN, V., MILNER, A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonants. Clinical Science. 26, 265?277, 1993 MOITA, J.M.N.; MOITA, G.C. Uma introdu??o ? an?lise explorat?ria de dados multivariados. Qu?mica.Nova, 21, 467-469, 1998. NACZK, M.; SHAHIDI, F. Extraction and analysis of phenolics in food. Journal of Chromatography A. 1054, 95-111, 2004 NOBUSHI, Y.; OIKAWA, N.; OKAZAKI, Y.; TSUTSUMI, S.; PARK, Y.K.; KUROKAWA, M.; YASUKAWA, K. Determination of Artepillin-C in Brazilian Propolis by HPLC with Photodiode Array Detector . Journal of Pharmacy and Nutrition Sciences. 2, 127-131, 2012. NUNES, C. A. ; GUERREIRO, M. C. Characterization of Brazilian green propolis throughout the seasons by Headspace-GC/MS and ESI-MS. Journal of the Science of Food and Agriculture. 92, 433-438, 2012. OLIVEIRA, A.C.P.; SHINOBU, C.S.; LONGHINI, R.; FRANCO SL, SVIDZINSKI, T.I.E. Antifungal activity of propolis extract against yeasts isolated from onychomycosis lesions. Mem?rias do Instituto Oswaldo Cruz. 101, 493-497, 2006. OLIVEIRA, G.L.S. Determina??o da capacidade antioxidante de produtos naturais in vitro pelo m?todo do DPPH?: estudo de revis?o. Revista. Brasikeira de Plantas. Medicinais.1, 36-44, 2015. ONLEN, Y.; TAMER, C.; OKSUZ, H.; DURAN, N.; ALTUG, M.E.; YAKAN, S. Comparative trial of different anti-bacterial combinations with propolis and ciprofloxacin on Pseudomonas keratitis in rabbits. Microbiological Research 162, 62-68, 2007. OPSENICA, D.M.; RISTI OJE I ,P.; TRIFKO I , J.; O K, I.; L I , D.; AND TE I , Z. TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis. Journal of Chromatographic Science, 29, 1-7; 2016. OTA, C.; UNTERKIRCHER, C.; FANTINATO, V.; SHIMIZU, M.T. Antifungal activity of propolis on different species of Candida. Mycoses. 44, 375-8, 2001. 153 PAPOTTI, G., BERTELLI, D., ROSSI, M.C., PLESSI, M. Use of HR-NMR to classify pr?polis obtained using different harvesting methods. International Journal of Food Science and Technology, 45, 1610?1618, 2010. PARK, Y.K, ALENCAR, S.M, AGUIAR, C.L. Botanical origin and chemical composition of Brazilian pr?polis. Journal of Agricultural and Food Chemistry. 50, 2502-2506, 2002. PARK, Y.K., PAREDES-GUZMAN, J.F., AGUIAR, C.L., ALENCAR, S.M., FUJIWARA, F.Y. Chemical constituents in Baccharis dracunculifolia as the main botanical origin of southeastern Brazilian propolis. Journal of Agricultural and Food Chemistry. 52, 1100-1103, 2004. PARK, Y.K.; ALENCAR, S.M.; AGUIAR, C.L. PAREDES-GUSM?N, J. Composi??o qu?mica de Baccharis dracunculifolia, fonte bot?nica das pr?polis dos estados de S?o Paulo e Minas Gerais. Ci?ncia Rural, 35, 909-915, 2005. PARK, Y.K.; KOO, M.H.; ABREU, J.A.; IKEGAKI, M.; CURY, J.A.; ROSALEN, P.L. Antimicrobial activity of propolis on oral microorganisms. Current Microbiology. 36, 24-28, 1998. PARK, Y.K.; KUMAZAWA, S. Direct evidence for the plant origin of Brazilian propolis by the observation of honeybee behavior and phytochemical analysis. Chemical & Pharmaceutical Bulletin. 51, 740-742, 2003 PARK,Y.K.; IKEGAKI, M.; ALENCAR, S.M.; MOURA, F.F. Evaluation of Brazilian propolis by both physicochemical methods and biological activity. Honeybee Science. 21, 85-90, 2000. PASTANA, R. F.; VIEIRA, G. H. C; MACHADO, P. P. so da pr?polis no controle ?in vitro? do fungo Colletotrichum gloeosporioides causador da antracnose em berinjela. Revista de Agricultura Neotropical. 3, 12?15, 2016. PAVIANI, L.C.; SAITO, E.; DARIVA, C.; MARCUCCI, M.C.; S?NCHEZ-CAMARGO, A.P.; CABRAL, F.A. Supercritical CO2 extraction of raw propolis and its dry ethanolic extract. Brazilian Journal of Chemical Engineering. 29, 243-251, 2012. PEREIRA, A. S.; SEIXAS, F.R.M.S.; NETO, F. R.A. Pr?polis: 100 anos de pesquisa e suas perspectivas futuras. Quimiva. Nova, Vol. 25, No. 2, 321-326, 2002. PEREIRA, A.S.; PEREIRA A.F.M.; TRUGO, L.C.; AQUINO NETO, F.R. Distribution of quinic acid derivatives and other phenolic compounds in Brazilian propolis. Zeitschrift f?r Naturforschung C. 58, 590-593, 2003. PEREIRA, A.S.; NASCIMENTO, E.A.; AQUINO, N.F. Lupeol alkanoates in Brazilian propolis. Zeitschrift f?r Naturforschung C. 57, 721-726, 2002. PEREIRA, B.K. Disserta??o de Mestrado Avalia??o do efeito fotoprotetor de tr?s extratos de plantas da Ant?rtica por diferentes m?todos biol?gicos. Universidade Federal do Rio Grande do Sul, Porto Alegre, 2007. P?REZ, R. A.; IGLESIAS, M. T.; PUEYO, E.; GONZ?LEZ, M.; DE LORENZO, C. Amino Acid Composition and Antioxidant Capacity of Spanish Honeys. Journal of Agricultural and Food Chemistry. 55, 360-365, 2007 154 P?REZ-JIM?NEZ, J.; SAURA-CALIXTO, F. Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Research International. 39,791-800, 2006 PICCINELLI, A. L.; MENCHERINI, T.; CELANO, R.; MOUHOUBI, Z.; AZEDDINE TAMENDJARI, A.; AQUINO, R.P.; RASTRELLI, L. Chemical composition and antioxidant activity of Algerian pr?polis. Journal of Agricultural and Food Chemistry. 61, 5080-5088, 2013 POPOVA, M.P.; BANKOVA, V.S.; BOGDANOV, S.; TSVETKOVA, I.; NAYDENSKI, C.; MARCAZZAN, L.; SABATINI, A. Chemical characteristics of poplar type propolis of different geographic origin. Apidologie. 38, 306-306, 2007. PULIDO, R.; BRAVO, L.; SAURACALIXTO, F. Antioxidant activity of dietary as determined by a modified ferric reducing antioxidant power assay. Journal Agriculture and Food Chemistry. 48, 3396-3402, 2000 QUEIROZ, S.C.N.; COLLINS, C.H.; JARDIM, I.C.S.F. M?todos de extra??o e/ou concentra??o de compostos encontrados em Fluidos biol?gicos para posterior determina??o cromatogr?fica. Qu?mica Nova. 24, 68-76, 2001 RE, R., PELLEGRINI, N., PROTEGGENTE, A., PANNALA, A., YANG, M., RICE-EVANS, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radicals and Biology Medicine. 26, 1231?1237, 1999 RIGHI, A.A.; NEGRI, G.; SALATINO, A. Comparative chemistry of propolis from eight brazilian localities. Evidence-Based Complementary and Alternative Medicine. 267878, 14, 2013. RISTI OJE I , P., ANDRI , F., TRIFKO I , J., O K, I., STANISA LJE I , L., TE I ., Z., MILOJKO I -OPSENICA, D. Pattern recognition methods and multivariate image analysis in HPTLC fingerprinting of propolis extracts. Journal of Chemometrics. 28, 4, 301?310. 2014 ROBBINS, R.J. Phenolic acids in foods: an overview of analytical methodology. Journal of Agricultural and Food Chemistry, 51, 2866-2887, 2003 ROLEIRA, F.M.F.; SILVA, E.J.T.; VARELA, C.L.;COSTA, S.C.; SILVA, T.; GARRIDO, J.; BORGES, F. Plant derived and dietary phenolic antioxidants: Anticancer properties. Food Chemistry 183, 235?258, 2015. RUFINO, M, S, M.; ALVES, R, E.; BRITO, E, S.; MORAIS, S. M.; SAMPAIO, C. G.; P?REZ-JIM?NEZ, J.; SAURA-CALIXTO, F. D. Metodologia Cient?fica: Determina??o da Atividade Antioxidante Total em Frutas pela Captura do Radical Livre ABTS.+.Comunicado t?cnico online 128 ISSN 1679-6535. 2007. SAHINLER, N.; KAFTANOGLU, O. Natural product propolis: chemical composition. Natural Product Research, 19, 183-188, 2005. SALATINO A, TEIXEIRA EW, NEGRI G AND MESSAGE D, Origin and chemical variation of Brazilian pr?polis. Evidence-Based Complementary Alternative Medicine. 2, 33?38, 2005. 155 SALGUEIRO, F.B.; CASTRO, R.N. Compara??o entre a composi??o qu?mica e capacidade antioxidante de diferentes extratos de pr?polis verde. Qu?mica Nova, 1-8, 2016. SALGUEIRO, F.B.; LIRA, A.F.; RUMJANEK, V.M.; CASTRO, R.N. Phenolic composition and antioxidant properties of Brazilian honeys. Qu?mica Nova,1-6, 2014. SAWAYA, A.C.H.F.; CUNHA, I.B.S.; MARCUCCI, M.C. Analytical methods applied to diverse types of Brazilian pr?polis. Chemistry Central Journal. 5-27, 2011 SEBRAE. Boletim: O Mercado da pr?polis. http://www.sebrae2014.com.br ? acessado em 11/12/14. SEELEY, T. D. The wisdon of the hive ? The social phisiology of honey bee colonies. Harvard: Harvard University Press. 1995. 295 p. SFORCIN, J. M.; BANKOVA, V. Propolis: is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133, 253-260, 2011 SFORCIN, J.M., DE SOUSA, J.P.R.; SILVA-FILHO, A.A.; BASTOS, J.K.; B?FFALO, M. C.; TONUCI, L.R.S.; Baccharis dracunculifolia. Uma das principais fontes vegetais da pr?polis brasileira. Editora UNESP, 2012. SHIMIZU, K. et al. Antioxidative bioavailability of artepillin C in Brazilian propolis. Archives of Biochemistry and Biophysics. 424, 181-188, 2004. S?L?C?, S.; KOC, A.; MISTIK, S. Comparsion of in vitro activities of antifungal drugs and propolis against yeasts isolated from patients with superficial mycoses. Annals of Microbiology. 57, 269, 2007. SILVA, C.C.F. Disserta??o de Mestrado . An?lise qu?mica e atividade antioxidante e citot?xica de amostras de pr?polis de alecrim. Universidade de S?o Paulo, 2008. SILVA, D.C.; CERCHIARO, G.; HON?RIO, M.K. Rela??es patofisiol?gicas entre estresse oxidativo e arteriosclerose. Qu?mica Nova. 34, 300-305, 2011. SILVA, R.A., RODRIGUES, A.E., RIBEIRO, A.C.M., CUST?DIO, A.R., ANDRADE, N.E.D., PEREIRA, W.E. Caracter?sticas f?sico-qu?micas e atividade antimicrobiana de extratos de pr?polis da Para?ba, Brasil. Ci?ncia Rural.. 36, 1842-1848, 2006. SILVA-CARVALHO, R.; BALTAZAR, F.; ALMEIDA-AGUIAR, C. Propolis: a complex natural product with a plethora of biological activities that can be explored for drug development. Evidence-Based Complementary and Alternative Medicine. 206439. 29, 2015. SILVERSTEIN,R.M.; WEBSTER, F.X.; KIEMLE, D. Identifica??o Espectrom?trica de Compostos Org?nicos, 2015. 7? Ed, LTC SIQUEIRA, A.B.;GOMES, B.S.; CAMBUIM, I.; MAIA, R.; ABREU, S.; SOUZA-MOTTA, C.M.; QUEIROZ, L.A.; PORTO, A.L. Trichophyton species susceptibility to green and red propolis from Brazil. Letters in Applied Microbiology. 48, 90-96, 2009. 156 SIRIPATRAWAN, U.; VITCHAYAKITTI, W.; SANGUANDEEKUL, R. Antioxidant and antimicrobial properties of Thai pr?polis extracted using ethanol aqueous solution. International Journal of Food Science and Technology. 48, 22?27, 2013. SIRVEN, J.; SALL?, B.; MAUCHIEN, P.; LACOUR, J.;MAURICE, S.; MANH?S, G. Feasibility study of rock identification at the surface of Mars by remote laser-induced breakdown spectroscopy and three chemometric methods. Journal of Analytical Atomic Spectrometry. 22, 1471-1480, 2007. SNYDER, L.R.; CARR, P.W.; RUTAN, S.C. Solvatochromically based solvent-selectivity triangle. Journal of Chromatography A. 656, 537-547, 1993 SOARES, S. E. ?cidos fen?licos como antioxidantes. Revista de Nutri??o.15, 71-81, 2002 SOUSA, J.P.B.; FURTADO, N.A.J.C; JORGE, R.; SOARES, A.E.E.; BASTOS, J.K. Perfis f?sico-qu?mico e cromatogr?fico de amostras de pr?polis produzidas nas microrregi?es de Franca (SP) e Passos (MG), Brasil. Brazilian Journal of Pharmacognosy.17, 85-93, 2007. SROKA, Z.; CISOWSKI, W. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food and Chemical Toxicology, 41, 753-758, 2003. STALIKAS, C. D. Review: Extraction, separation, and detection methods for phenolic acids and flavonoids . Journal of Separation Science. 30, 3268 ? 3295, 2007. STEELS, E.L.; LEARMONTH, R.P.; WATSON, K. Stress tolerance and membrane lipid unsaturation in Saccharomyces cerevisiae grown aerobically or anaerobically. Midiology. 140, 569-576, 1994. STEPANOVIC, S.; ANTIC N, DAKIC I, VLAHOVIC, M.S. In vitroantimicrobial activit of propolis and synergism between propolis and antimicrobial drugs. Microbiological Research. 158, 353-7, 2003. SULAIMAN, G.M; AL SAMMARRAE, K.W.; ADHIAH, A.H.; ZUCCHETTI, M.; FRAPOLLI, R.; BELLO, E.; ERBA, E.; D?INCALCI, M.; BAGNATI, R. Chemical characterization of Iraqi pr?polis samples and assessing their antioxidant potentials. Food and Chemical Toxicology. 49, 2415?2421, 2011. SZLISZKA, E.; ZENON, P,A.M.;. CZUBA, KR?L, W. Inhibition of Inflammatory Response by Artepillin C in Activated RAW264.7 Macrophages. Evidence-Based Complementary and Alternative Medicine. 735176, 11, 2013. TANG, T.X.; GUO, W.Y.; XU, Y.; ZHANG, S.M.; XU, X.J.; WANG, D.M.; ZHAO, Z.M.; ZHU, L.P.;YANG, D.P. Thin-layer chromatographic identification of Chinese propolis using chemometric fingerprinting. Phytochemical Analysis. 25, 266-72, 2014. TAZAWA, S.; WARASHINA, T.; NORO, T.; MIYASE, T. Studies on the constituents of Brazilian propolis. Chemical and Pharmaceutical Bulletin.46, 1477-1479, 1998. TEIXEIRA, E.W.; MESSAGE, D.; MEIRA, R.M.S.A.; SALATINO, M. Indicadores da origem bot?nica da pr?polis: import?ncia e perspectivas. Boletim da Ind?stria Animal, 60, 83-106, 2003. 157 THIRUGNANASAMPANDAN, R.; RAVEENDRAN, S.B.; JAYAKUMAR, R. Analysis of chemical composition and bioactive property evaluation of Indian pr?polis. Asian Pacific Journal of Tropical Biomedicine. 651-654, 2012. TOMAZZOLI, M.M.; PAI NETO, R.D.; MORESCO, R.; WESTPHAL, L.; ZEGGIO, A.R.; SPECHT, L.; COSTA, C.; ROCHA, M.; MARASCHIN, M.. Discrimination of Brazilian propolis according to the seasoning u |
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