The structure of the capsular polysaccharides from
Klebsiella serotype K60 and K26 have been determined using
the techniques of methylation.periodate oxidation,partial
hydrolysis, and β-elimination. ¹H- and ¹³C-n.m.r. spectroscopy
was used to establish the nature of the anomeric linkages
in both polysaccharides and also in the oligosaccharides
obtained by the different degradative techniques used.
Specific hydrolases obtained from bacteriophages were utilized to degrade two Klebsiella polysaccharides. Larger quantities of oligosaccharide repeating units can be generated
in this manner. Two bacteriophages, Ø60 and Ø46,the first one with β-glucosidase activity and the other with
β-galactosidase activity, were used to degrade the corresponding
polysaccharides according to a new, simplified procedure.
The purified gum exudate from Chorisia speciosa (palo borracho) was studied. The results from methylation analysis,
β-elimination and partial hydrolysis made possible a tentative assignment for an "average structure" of the gum polysaccharide. / Science, Faculty of / Chemistry, Department of / Graduate
| Identifer | oai:union.ndltd.org:UBC/oai:circle.library.ubc.ca:2429/22810 |
| Date | January 1981 |
| Creators | Di Fabio, Jose Luis |
| Source Sets | University of British Columbia |
| Language | English |
| Detected Language | English |
| Type | Text, Thesis/Dissertation |
| Rights | For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. |
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