The chromatographic behaviour of a new column packing for high performance size exclusion chromatography based on crosslinked polyacrylamide particles was studied. Experimental retention data for poly(ethylene glycol)and poly(ethylene oxide) standards in water and water-methanol mixtures showed that the separation mechanism is size exclusion, since a universal calibration plot based on hydrodynamic volume was obtained. For polysaccharide standards in water and water-methanol (80/20), the universal calibration was valid for molecular weights above 4 * 10~. Below this value, secondary mechanisms appear to be taking part in the separation since water-methanol (80/20) is a poor solvent for polysaccharides. Crosslinked polyacrylamide packings showed an interactive behaviour with tetrahydrofuran and dimethylformamide as eluents with polystyrene and poly( ethylene glycol )/poly(ethylene oxide) standards and the universal calibration was not valid.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:374470 |
| Date | January 1986 |
| Creators | Montenegro, Ana M. C. |
| Publisher | Loughborough University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | https://dspace.lboro.ac.uk/2134/11754 |
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