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Microarray Analysis Of The Effects Of Heat And Cold Stress On Hydrogen Production Metabolism Of Rhodobacter Capsulatus

Rhodobacter capsulatus DSM1710 is a purple non-sulfur bacterium capable of
hydrogen production via photofermentation. Biohydrogen is a clean and renewable
way of hydrogen production, which can be achieved by PNS bacteria in outdoor
large scale photobioreactors using sun light. In outdoor conditions bacteria can be
exposed to heat and cold stress. In this study in order to understand the effects of heat
and cold stress on photofermentative hydrogen production and gene expression
profile of R.capsulatus on acetate as the carbon source, microarray analysis was
carried out. Since there is no commercially available microarray chip for
R.capsulatus, an Affymetrix GeneChip&reg / was designed and it was manufactured by
Affymetrix.The experiments were conducted at 30

Identiferoai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12613668/index.pdf
Date01 September 2011
CreatorsGurgan Dogan, Muazzez
ContributorsYucel, Meral
PublisherMETU
Source SetsMiddle East Technical Univ.
LanguageEnglish
Detected LanguageEnglish
TypeM.S. Thesis
Formattext/pdf
RightsAccess forbidden for 1 year

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