Prominin-1 is a lipid raft–associated, cholesterol-binding membrane glycoprotein selectively associated with plasma membrane protrusions and extracellular vesicles derived therefrom. Despite its worldwide use for stem cell isolation and its clinical importance in cancer-initiating cells and photoreceptor morphogenesis the function of prominin-1 remains elusive. This prompted me to investigate its role in the architecture and dynamics of microvilli and primary cilia at the apical plasma membrane of Madin-Darby canine kidney (MDCK) cells. Therefore, stably transfected cell lines were established expressing human prominin-1 splice variant 1 or 2.
Upon the overexpression of prominin-1 the number of individual microvilli and clusters of them increased significantly. I also noticed alterations in their architecture, i.e. branching microvilli. Fascinatingly, two point mutations (Pro37→Ala and Tyr41→Ser) in the ganglioside GM1-binding motif of prominin-1 increased the number of branched microvilli and generated irregular ones with knob-like structures at their tip. Additionally, the release of prominin-1+ vesicles was impaired. Interestingly, both phenotypes were suppressed by the inhibition of the phosphoinositide 3-kinase (PI3K) or the Arp2/3 complex. Impaired interaction of prominin-1 with the PI3K through the introduction of an additional mutation (Tyr828→Phe) in its PI3K-binding site also reduced the amount of structurally altered microvilli. Thus, the interaction of prominin-1 with the PI3K may drive the conversion of the docking phospholipid phosphatidylinositol(4,5)-bisphosphate into phosphatidylinositol(3,4,5)-trisphosphate resulting in the uncoupling of the microvillar membrane from the underlying actin filaments thereby creating irregular/knob-like microvilli. Simultaneously, the phospholipid conversion might modulate the activity of regulators and/or activators of the Arp2/3 complex leading to the branching of microvilli.
The overexpression of human prominin-1 also increased the length of primary cilia. Remarkably, a mutation in the histone deacetylase 6-binding site that mimics acetylation produces shorter cilia in cells expressing human prominin-1.s2. Additionally, it stimulates membrane vesicle release and dome formation. Above these striking observations, I observed branching cilia and cilia with a pearling shape.
Collectively, the data suggest that a complex interplay of prominin-1 with its lipid and protein interaction partners regulates the architecture and dynamics of cellular protrusions.
Finally, a growing number of studies use canine prominin-1 as an antigenic marker despite the absence of specific antibodies. Studies investigating its expression in dog tissues or cells derived therefrom rely on antibodies directed against its human and murine orthologs. To determine its cross-species immunoreactivity I cloned canine prominin-1 and overexpressed it as a green fluorescent protein fusion protein in MDCK cells. Here, I show that the genomic structure of the canine prom1 gene is similar to that of human and mouse. Canine prominin-1 shows the common characteristics of the prominin-1 family but the primary structure is poorly conserved. Like human and mouse protein, it is targeted to the apical membrane of MDCK cells and specifically enriched in microvilli and primary cilia. Immunocytochemistry, flow cytometry and immunoblotting techniques revealed that none of the applied antibodies against human or mouse prominin-1 recognizes the canine protein.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:30742 |
| Date | 15 January 2018 |
| Creators | Thamm, Kristina |
| Contributors | Corbeil, Denis, Hoflack, Bernard, Simons, Kai, Technische Universität Dresden |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | doc-type:doctoralThesis, info:eu-repo/semantics/doctoralThesis, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0023 seconds