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Previous issue date: 2018-01-10 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / (Artigo 1 Efeito das varia??es do pH na superf?cie de resinas compostas de incremento ?nico)
Aim: To evaluate, in vitro, quantitatively and qualitatively, the effect of pH
variations on surface roughness (Ra) and bacterial adhesion (Cn) of Bulk-Fill
composite resins followed by simulated toothbrushing. Materials and Methods:
Thirty samples of each composite resin were obtained: G1 (control): Z250; G2:
Filtek Bulk-Fill (FTK); G3: Tetric N Ceram Bulk-Fill (TTC); G4: Aura Bulk-Fill
(AUR). After 24 h, the samples were polished and then alternated by DES/RE
solutions for 15 cycles of 24 h each at 37?C. Then, the samples were submitted
to simulated toothbrushing. The Ra and Cn measurements were quantitatively
analyzed in three stages: after polishing (Ra0 and Cn0), after pH cycles (Ra1 and
Cn1) and after simulated toothbrushing (Ra2 and Cn2). Measurements on new
samples submitted only to simulated toothbrushing were also performed (Ra* and
Cn*). The Ra values were submitted to two-way ANOVA, followed by the Tukey?s
test (?=0.05). The Kruskal-Wallis test followed by multiple comparisons was
applied for Cn analysis. Surface topography and bacterial adhesion were
observed by Scanning Electron Microscopy (SEM). Results: Z250, FTK and TTC
showed no significant change in Ra regardless of the treatment performed,
except for AUR which obtained superior Ra at Ra2 (p <0.05). In the bacterial
count, FTK differed from the others in Cn0 and Cn1. In Cn2 there was no
difference between the composite resins. Only after Cn*, the Z250 differed from
all other materials. SEM images showed cracking and exposure of fillers in Ra1,
with detachment of the exposed fillers. Microcavities were observed in all
composite resins in Ra2. Bacterial adhesion occurred in all composite resins
regardless of the treatment. There was greater bacterial adhesion in Cn1 for Z250
and FTK. Conclusion: The pH cycles did not alter the Ra of composite resins
Z250, FTK, TTC and AUR. Simulated toothbrushing after pH cycles caused Ra
increase only for AUR, and reduced bacterial adhesion in Z250 and FTK
composite resins. There was degradation of the surface of all composite resins,
as well as bacterial adhesion.
(Artigo 2 Effect of gastric acid on the surface of bulk-fill composite resins)
Aim: To evaluate, in vitro, quantitatively and qualitatively, the effect of gastric
acid on surface roughness and bacterial adhesion in Bulk-Fill composite resins
followed by simulated toothbrushing. Materials and Methods: 30 samples of
each Bulk-Fill composite resin were obtained: G1 (control): Z250; G2: Filtek Bulk-
Fill (FTK); G3: Tetric N-Ceram Bulk-Fill (TTC); G4: Aura Bulk-Fill (AUR). The
samples were polished after 24 h and immersed in 10 ml of gastric acid for 24 h
at 37?C. Following, the samples were submitted to simulated toothbrushing. Ra
and Cn were obtained in three different stages: initial (Ra0 and Cn0), after gastric
acid (Ra1 and Cn1) and after gastric acid and simulated toothbrushing (Ra2 and
Cn2). Additional samples were submitted to the simulated toothbrushing test only
and received the same measurements (Ra* and Cn*).Two-way ANOVA was used
to evaluate Ra, followed by the Tukey test. Regarding the Cn analysis, the
Kruskal-Wallis test was used, followed by multiple comparisons. The significance
level was of 5%. Results: Z250 and FTK had a significant increase in surface
roughness in Ra1. In Ra2, the four composite resins did not significantly differ
from Ra0, but there was a significant reduction of the means in Ra2 of Z250 and
FTK compared to Ra1. For Cn, Z250 and FTK allowed an increased biofilm
accumulation in comparison with TTC and AUR. SEM images showed crack
formation and exposure of fillers in Ra1, occurring detachment of the exposed
fillers. Microcavities were observed in all composite resins in Ra2. Bacterial
adhesion occurred in all composite resins regardless of the treatment. There was
higher bacterial adhesion in Cn1, followed by reduction of bacterial adhesion in
Cn2 for all tested materials, except for AUR. Conclusion: Gastric acid increased
the surface roughness of Z250 and FTK composite resins, as well as promoted
higher bacterial accumulation compared to TTC and AUR composite resins.
There was degradation of the surface of all composite resins, as well as bacterial
adhesion. / (Artigo 1 Efeito das varia??es do pH na superf?cie de resinas compostas de incremento ?nico)
Objetivo: Avaliar, in vitro, de forma quantitativa e qualitativa, o efeito das
varia??es de pH na rugosidade superficial (Ra) e na ades?o bacteriana (Cn) de
resinas compostas de incremento ?nico seguido de escova??o simulada.
Materiais e M?todos: Trinta amostras de cada resina composta (5mm de largura
e 4mm de altura) foram obtidas -G1(controle): Z250; G2: Filtek Bulk-Fill (FTK);
G3: Tetric N Ceram Bulk-Fill (TTC); G4: Aura Bulk-Fill (AUR). Ap?s 24 h, as
amostras foram polidas e ent?o alternaram por solu??es DES/RE por 15 ciclos
de 24 h cada a 37?C. Na sequ?ncia foram submetidas ? escova??o simulada. As
medidas Ra e Cn foram analisadas quantitativamente em tr?s etapas: ap?s
polimento (Ra0 e Cn0), ap?s ciclos de pH (Ra1 e Cn1) e ap?s escova??o
simulada (Ra2 e Cn2). Medi??es em novas amostras submetidas apenas ?
escova??o simulada foram realizadas (Ra* e Cn*). Para an?lise dos dados de
Ra foi utilizada a ANOVA de dois fatores, seguida do teste de Tukey (?=0,05). O
teste de Kruskal-Wallis seguido de compara??es m?ltiplas foi utilizado para
an?lise de Cn. A topografia superficial e a ades?o bacteriana foram observadas
em Microscopia Eletr?nica de Varredura (MEV). Resultados: Z250, FTK e TTC
n?o tiveram altera??o significativa de Ra independente do tratamento realizado,
com exce??o da AUR que obteve Ra superior em Ra2 (p<0,05). Em Cn, FTK
diferiu das demais em Cn0 e Cn1. Em Cn2 n?o houve diferen?a entre as resinas
compostas. Apenas ap?s Cn*, a Z250 diferiu dos outros materiais. Imagens em
MEV evidenciaram forma??o de trincas e exposi??o de part?culas de carga em
Ra1, com desprendimento das part?culas expostas. Houve forma??o de
microcavidades em todas as resinas compostas em Ra2. Ocorreu ades?o
bacteriana em todas as resinas compostas independente do tratamento. Houve
maior ades?o bacteriana em Cn1 para Z250 e FTK. Conclus?o: Os ciclos de pH
n?o alteraram Ra das resinas compostas Z250, FTK, TTC e AUR. A escova??o
simulada ap?s os ciclos de pH causou aumento de Ra apenas para AUR e
reduziu a ades?o bacteriana nas resinas compostas Z250 e FTK. Houve
degrada??o da superf?cie de todas as resinas compostas, assim como ades?o
bacteriana.
(Artigo 2 Efeito do ?cido g?strico na superf?cie de resinas compostas de incremento ?nico)
Objetivo: Avaliar de forma quantitativa e qualitativa o efeito do ?cido g?strico na
rugosidade superficial e na ades?o bacteriana nas resinas compostas de
incremento ?nico (Bulk-Fill) seguido de escova??o simulada. Materiais e
M?todos: Trinta amostras de cada resina composta (di?metro de 5mm e altura
de 4mm) foram obtidas - G1(controle): Z250; G2: Filtek Bulk-Fill (FTK); G3: Tetric
N-Ceram Bulk-Fill (TTC); G4: Aura Bulk-Fill (AUR). As amostras foram
confeccionadas e polidas ap?s 24 h. As amostras foram imersas em 10 ml de
?cido g?strico durante 24 h em 37?C. Na sequ?ncia, as resinas compostas foram
submetidas ? escova??o simulada. As medidas de Rugosidade Superficial (Ra)
e Contagem bacteriana (Cn) foram obtidas em tr?s diferentes etapas: inicial (Ra0
e Cn0), ap?s ?cido g?strico (Ra1 e Cn1) e ap?s ?cido g?strico e escova??o
simulada (Ra2 e Cn2). Amostras adicionais foram submetidas unicamente ao
teste de escova??o simulada e receberam as mesmas medi??es (Ra* e Cn*).
ANOVA de dois fatores foi utilizada para avalia??o de Ra, seguida do teste de
Tukey. Para an?lise de Cn foi utilizado o teste de Kruskal-Wallis, seguido de
compara??es m?ltiplas. O n?vel de signific?ncia foi de 5%. A topografia
superficial e a ades?o bacteriana foram observadas em microscopia eletr?nica
de varredura (MEV). Resultados: Z250 e FTK tiveram aumento significativo na
rugosidade superficial em Ra1. Em Ra2, as quatro resinas compostas n?o
diferiram estatisticamente de Ra0, por?m houve redu??o significativa das m?dias
em Ra2 da Z250 e FTK em compara??o a Ra1. Para Cn, Z250 e FTK permitiram
maior ac?mulo de biofilme em rela??o ? TTC e AUR. Imagens em MEV
evidenciaram forma??o de trincas e exposi??o de part?culas de carga em Ra1,
ocorrendo desprendimento das part?culas de carga expostas. Microcavidades
foram observadas em todas as resinas compostas em Ra2. A ades?o bacteriana
ocorreu em todas as resinas compostas independente do tratamento. Houve
maior ades?o bacteriana em Cn1, seguida de redu??o da ades?o bacteriana em
Cn2 para todos os materiais testados, exceto pela AUR. Conclus?o: O ?cido
g?strico aumentou a rugosidade superficial das resinas compostas Z250 e FTK,
assim como promoveu maior ac?mulo bacteriano em rela??o ?s resinas
compostas TTC e AUR. Houve degrada??o da superf?cie de todas as resinas
compostas, assim como ades?o bacteriana.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7896 |
| Date | 10 January 2018 |
| Creators | Somacal, Deise Caren |
| Contributors | Spohr, Ana Maria |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, Brasil, Escola de Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 500, 600, -2070498469879244349, 2075167498588264571 |
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