Amiloride, a diuretic used for managing resistant hypertension, is hindered by low oral bioavailability and Gastrointestinal side effects, impacting patient compliance. This study investigates transdermal delivery as an alternative to reduce pill burden and improve adherence. Using dermatomed porcine ear skin, the research examined the passive transdermal delivery of amiloride and the effect of various chemical enhancers, including oleic acid, oleyl alcohol, tocopheryl polyethylene glycol succinate, and N-methyl-2-pyrrolidone, individually or in combination. A validated High-Performance Liquid Chromatography method, adhering to Internal Council for Harmonized guidelines (ICH), was employed for quantitative analysis. In vitro permeation studies, conducted using porcine skin and Franz diffusion cells over 168 hours, revealed that oleyl alcohol (761.86±74.97 µg/cm²) and oleic acid (691.90±78.59 µg/cm²) were the most effective enhancers. These findings suggest that chemical enhancers can significantly facilitate the transdermal delivery of amiloride, offering a promising alternative to enhance patient compliance and achieve better therapeutic outcomes.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etd-5976 |
| Date | 01 August 2024 |
| Creators | Leshaodo, Oluwatosin Tabitha |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Electronic Theses and Dissertations |
| Rights | Copyright by the authors. |
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