Cheng, Cho Yiu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 186-217). / Abstracts in English and Chinese. / Acknowledgements --- p.1 / Abstract of thesis --- p.2 / Abstract of thesis in Chinese --- p.7 / Presentation attended --- p.9 / Chapter Chapter 1: --- Introduction and Background --- p.13 / Chapter 1.1 --- Anatomy and functions of human prostate gland --- p.13 / Chapter 1.2 --- Worldwide epidemiology of prostate cancer --- p.15 / Chapter 1.3 --- Prostate cancer stages and treatments in clinic --- p.21 / Chapter 1.4 --- Introduction to nuclear receptors --- p.23 / Chapter 1.5 --- Nuclear receptor structure --- p.24 / Chapter 1.6 --- Nuclear receptors nomenclature and classification --- p.28 / Chapter 1.7 --- Mode of action for nuclear receptors --- p.34 / Chapter 1.8 --- Co-regulators of nuclear receptors --- p.35 / Chapter 1.9 --- Nuclear receptors related to prostate cancer --- p.43 / Chapter Chapter 2: --- Aim of study and experimental design --- p.59 / Chapter 2.1 --- Aim of study --- p.59 / Chapter 2.2 --- In vitro cell lines models used in the study --- p.60 / Chapter Chapter 3: --- Materials and methods --- p.64 / Chapter 3.1 --- Apparatus and preparation throughout the study --- p.64 / Chapter 3.2 --- Cells culture --- p.64 / Chapter 3.3 --- RNA extraction --- p.67 / Chapter 3.4 --- Reverse transcription --- p.68 / Chapter 3.5 --- Primers specificity checking --- p.69 / Chapter 3.6 --- Real time quantitative polymerase chain reaction --- p.84 / Chapter 3.7 --- Data analysis --- p.90 / Chapter Chapter 4: --- Results --- p.92 / Chapter 4.1 --- Expression of nuclear receptors transcripts in each prostatic cell lines used --- p.92 / Chapter 4.2 --- Expression of nuclear receptor transcripts in immortalized prostatic epithelial BPH-1 and BPH-1 derived cell lines model --- p.116 / Chapter 4.3 --- Expression of nuclear receptor transcripts in androgen-dependent and androgen-independent classical prostatic cancer cell lines model --- p.121 / Chapter 4.4 --- Expression of nuclear receptor transcripts in androgen-independent and antiandrogen-resistant LNCaP derived cell lines model --- p.125 / Chapter Chapter 5: --- Discussion --- p.129 / Chapter 5.1 --- Special expression pattern of some nuclear receptors in the prostatic cell lines or prostatic cancer cell lines --- p.129 / Chapter 5.2 --- BPH-1 and BPH-1 derived cell lines model --- p.138 / Chapter 5.2.1 --- Prostatic cell lines model studying the transformation and invasion in prostate cancer (BPH-1 Snail & BPH-1 CAFTDs versus BPH-1) --- p.138 / Chapter 5.2.2 --- Prostatic cell lines model studying the transformation and invasion in prostate cancer (BPH-1 Snail & BPH-1 CAFTDs versus BPH-1 AR) --- p.159 / Chapter 5.3.3 --- classical prostatic cancer cell lines model --- p.162 / Chapter 5.3.1 --- Prostatic cancer cell lines model studying androgen-dependence and androgen-independence (DU145 & PC-3 versus LNCaP) --- p.163 / Chapter 5.4 --- LNCaP and LNCaP derived cell lines model --- p.170 / Chapter 5.4.1 --- Prostatic cancer cell lines model studying androgen-independence and antiandrogen-resistance (LNCaP-abl & LNCaP-BCs versus LNCaP) --- p.171 / Chapter Chapter 6: --- Conclusion --- p.179 / References --- p.186
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327469 |
| Date | January 2011 |
| Contributors | Cheng, Cho Yiu., Chinese University of Hong Kong Graduate School. Division of Biomedical Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, 217 leaves : ill. (chiefly col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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