Functional immobilization and lateral organization of proteins into micro- and nanopatterns is an important prerequisite for miniaturizing analytical and biotechnological devices. In this thesis I present novel and versatile approaches for high contrast surface micropatterning of proteins, artificial membranes and live cells based on maleimide photochemistry. The patterning strategy is carried-out on glass substrates exploiting a poly(ethylene glycol) PEG polymer layer as a compatible scaffold. The flexible PEG cushion prevents unspecific proteins attachment and cell adhesion to surfaces. The versatility of this method is demonstrated by means of different orthogonal chemistries using covalent- and affinity- based interactions for protein immobilization. Furthermore, using maleimide based alkyl-thiol chemistry, I utilized the patterning approach for capturing liposomes and proteoliposomes onto surfaces. Formation of fluid patterned polymer-supported membranes demonstrating lateral diffusion of lipids and proteins was confirmed by biophysical assays. A similar approach was used for micropatterning of transmembrane proteins in surface adhered live cells.
Identifer | oai:union.ndltd.org:uni-osnabrueck.de/oai:repositorium.ub.uni-osnabrueck.de:urn:nbn:de:gbv:700-2012101510347 |
Date | 15 October 2012 |
Creators | Waichman, Sharon |
Contributors | Prof. Dr. Jacob Piehler, Prof. Dr. Heinz-Jürgen Steinhoff |
Source Sets | Universität Osnabrück |
Language | English |
Detected Language | English |
Type | doc-type:doctoralThesis |
Format | application/pdf, application/zip |
Rights | http://rightsstatements.org/vocab/InC/1.0/ |
Page generated in 0.0014 seconds