This study used polyacrylamide gel electrophoresis to study the acid-phenol soluble proteins of five strains (A. vinelandii 12837, A. vinelandii 0, A. chroococcum 8004, A. macrocytogenes 8702, A. tumefaciens) of bacteria grown on Burk's nitrogen-free media, Trypticase Soy Broth, and 0.3% butanol medium. The results showed that the protein patterns can be used for the identification and possibly the taxonomic classification of the Azotobacter. The change of phenotype of the bacteria in different media followed the change of protein quantity and quality. There was no absolute similarity between any two of the species studied and this suggests a genetically heterogenous group of organisms while the amount of common proteins suggests close genetic relationships. Further studies are necessary to confirm the status of A. tumefaciens.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc663344 |
| Date | 08 1900 |
| Creators | Hsu, Li-Chu Yao |
| Contributors | Vela, G. Roland, 1927-, Gracy, Robert W. |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | 52 leaves: ill., Text |
| Rights | Public, Hsu, Li-Chu Yao, Copyright, Copyright is held by the author, unless otherwise noted. All rights |
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