Interest in flaxseed and products derived from flaxseed has increased considerably in the past decade. In the development of nutraceutical foods, several flaxseed components including lignans and alpha-linolenic acid, have been recognized to have health benefits. There are many patents and health claims to these components; however, relatively little research and information is found on flaxseed proteins. The objective of the present study was to isolate and characterize flaxseed proteins from defatted flaxseed meal. / Proteins were extracted from defatted flaxseed meal with NaOH. NaCl and NaCl/Papain. Protein solubility with these extractants ranged from 18 to 25%. Proteins were precipitated from the extracts using one or more of the following precipitation techniques; isoelectric precipitation (IP) gave yields and protein contents ranging between 22--25% and 67--73% respectively. Co-precipitation with soy and whey proteins gave yields of 26.95 and 35.78% respectively for a NaOH extract. Chemical hydrolysis of flaxseed meal with NaOH, NaCl and NaCl/Papain extraction and IP increased protein solubilization (35--43%) and protein yields (19--37%). / Proteins in the extracts and isolates were characterized by polyacrylamide gel electrophoresis (PAGE). Proteins extracted with NaOH and NaCl gave two bands by native-PAGE with molecular weights (MW) of 320 and 514KDa; proteins extracted with NaCl/Papain gave a single band at 188KDa. SDS-PAGE of isolates extracted with NaOH, NaCl and NaCl/Papain gave subunits with MW ranging from 6.5 to 56KDa. The major fractions isolated from NaOH-IP by RP-HPLC showed subunits with MW ranging from 6.5 to 40.1KDa by SDS-PAGE and 5.9 to 42.5KDa by ESI-MS. Subunits characterized by ESI-MS have not been reported previously in the literature.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.78355 |
| Date | January 2003 |
| Creators | El-Ramahi, Razan S. |
| Contributors | Alli, I. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Food Science and Agricultural Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001986326, proquestno: AAIMQ88190, Theses scanned by UMI/ProQuest. |
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