Porcine reproductive and respiratory syndrome virus (PRRSV) causes disease in swine and economic losses for swine producers. An inexpensive and effective method for removing PRRSV from air is required to reduce aerosol transmission of PRRSV. A laboratory study was used to assess the performance of air ionization at removing bioaerosols contaminated with PRRSV. Aerosol properties were measured with an Aerosol Particle Size Spectrometer and air samples were collected with SKC biosamplers. PRRSV RNA was quantified with RNA extraction and quantitative reverse transcription polymerase chain reaction. Reduction in aerosol concentrations ranged from 61 – 93% by number count and 68 – 96% by mass. Initial particle size distribution and airflow rate affected the performance of EPI Air at reducing aerosol concentrations. Air ionization was effective at removing PRRSV from the air. The PRRSV RNA concentrations were reduced by 68 – 90% and the average PRRSV RNA after ionization ranged from 154 – 4594 VGCN/m3.
Identifer | oai:union.ndltd.org:MANITOBA/oai:mspace.lib.umanitoba.ca:1993/30218 |
Date | 13 January 2015 |
Creators | La, Amy |
Contributors | Zhang, Qiang (Biosystems Engineering), Levin, David (Biosystems Engineering) Coombs, Kevin (Medical Microbiology) |
Source Sets | University of Manitoba Canada |
Detected Language | English |
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