Yes / Functional genomics research can give us valuable insights into bacterial gene
function. RNA Sequencing (RNA-seq) can generate information on transcript
abundance in bacteria following abiotic stress treatments. In this study, we used the
RNA-seq technique to study the transcriptomes of the opportunistic nosocomial
pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown
at both the human body temperature (37
C) and an arbitrarily-selected
temperature of 46
C. In this work using RNA-seq, we identified 133 genes that are
differentially expressed at 46
C compared to the human body temperature. Our
work identifies some key P. aeruginosa PAO1 genes whose products have importance
in both environmental adaptation as well as in vivo infection in febrile hosts. More
importantly, our transcriptomic results show that many genes are only expressed
when subjected to heat shock. Because the RNA-seq can generate high throughput
gene expression profiles, our work reveals many unanticipated genes with further
work to be done exploring such genes products. / University of Malaya High Impact Research (HIR) UM-MOHE HIR Grants (UM.C/625/1/HIR/MOHE/CHAN/14/1, No. H-50001-A000027; UM.C/625/1/HIR/MOHE/CHAN/01, No. A000001-50001); PPP Grant (PG081-2015B)
| Identifer | oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/15203 |
| Date | 2016 July 1919 |
| Creators | Chan, K., Priya, K., Chang, Chien-Yi, Abdul Rahman, A.Y., Tee, K.K., Yin, W. |
| Source Sets | Bradford Scholars |
| Language | English |
| Detected Language | English |
| Type | Article, Published version |
| Rights | © 2016 Chan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed., CC-BY |
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