It is well established that estrogens suppress feeding primarily by reducing meal size, and that this is partly mediated by enhancement of the response to satiation signals.
Glucagon-like peptide 1 (GLP-1) acts on receptor populations both peripherally and centrally to affect food intake. Most research on the feeding effects of GLP-1 has used exclusively male
subjects, and little is known about the effects of GLP-1 in females. We hypothesized that modulation of the central GLP-1 system is one of the mechanisms underlying the effects of
estrogens on ingestive behavior. More specifically, we hypothesized that estradiol, a common estrogen, enhances the anorexic response to central GLP-1. To investigate this possibility,
bilaterally ovariectomized female rats were placed on a cyclic regimen of either 2 μg β-estradiol-3-benzoate or oil vehicle and implanted with unilateral cannulas targeting the lateral
ventricle. We assessed the food intake effects of 0, 1, or 10 μg doses of GLP-1 in oil- or EB-treated rats administered 30 min prior to dark onset on the day following hormone treatment.
GLP-1 treatment significantly suppressed food intake in EB-treated rats at both doses compared to vehicle, whereas only the 10 μg dose was effective in oil-treated rats. We then examined
whether estrogen status alters the neuronal response to GLP-1 by measuring GLP-1-induced c-Fos expression in several feeding-relevant brain areas. While GLP-1 significantly increased c-Fos
expression, there were no significant differences between hormone treatment groups in the brain areas examined. These experiments suggest that modulation of the central GLP-1 system may be
one of the mechanisms by which estrogens suppress food intake and support the need for further examination of this effect. / A Thesis submitted to the Department of Psychology in partial fulfillment of the requirements for the degree of Master of Science. / Fall Semester 2015. / October 29, 2015. / c-Fos, estradiol, food intake, GLP-1, glucagon-like peptide 1, pharmacology / Includes bibliographical references. / Diana L. Williams, Professor Directing Thesis; Lisa A. Eckel, Committee Member; Pamela K. Keel, Committee Member.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_291372 |
| Contributors | Maske, Calyn B. (authoraut), Williams, Diana L. (professor directing thesis), Eckel, Lisa A. (committee member), Keel, Pamela K., 1970- (committee member), Florida State University (degree granting institution), College of Arts and Sciences (degree granting college), Department of Psychology (degree granting department) |
| Publisher | Florida State University |
| Source Sets | Florida State University |
| Language | English, English |
| Detected Language | English |
| Type | Text, text |
| Format | 1 online resource (35 pages), computer, application/pdf |
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