In this study, oligo-porphyrans, obtained by acid hydrolysis of porphyran, were investigated for their H2O2-inducing abilities in the defense responses of P. yezoensis. Oligo-porphyrans with average molecular weights (MWs) lower than 1.43 kDa had H2O2-inducing abilities. In contrast, oligo-porphyrans with average MWs of 6.12 kDa triggered no response. The active oligo-porphyrans were fractioned by anion-exchange chromatography. We found that two distinct mechanisms might be involved in the oligo-porphyran-induced H2O2 release in P. yezoensis. Mixtures of mono-sulfated oligo-galactans with degrees of polymerization (DPs) ranging from 1 to 3 might induce the response through the oxidation of cellular oligosaccharides, which enable P. yezoensis to resist rotting caused by dense incubation. Mixtures of oligo-porphyrans, consisting of 4 ~ 7 monosaccharide residues and 2 ~ 3 sulfate groups, might induce the generation of H2O2 by activation of NADPH oxidase, leading to an oxidative burst in P. yezoensis. The elicitor activity of oligo-porphyrans thus depends on their molecular size.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-16749 |
| Date | 01 January 2015 |
| Creators | Hou, Yun, Wang, Jing, Simerly, Thomas, Jin, Weihua, Zhang, Hong, Zhang, Quanbin |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | ETSU Faculty Works |
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