Nucleosomes comprise the most basic repeating unit of chromatin and provide hubs for the regulation of DNA transcription, replication and repair. ATPase chromatin remodelling complexes establish nucleosome occupancy, positioning and structure in a dynamic fashion to allow fine-tuning of protein-DNA interactions. The ISWI and CHD families of remodelers possess the ability to sample DNA linker length between nucleosomes and space nucleosomes evenly. How these spacing remodelers combine their functions to maintain phasing of nucleosomal arrays, and re-organise these arrays during development remains poorly understood. Furthermore the relationship between nucleosomal array structure and transcriptional regulation is unclear. Dictyostelium discoideum provides a complex chromatin environment and remodeler repertoire, while retaining a compact genome and ease of genetic manipulation. Thus we have utilized this model to generate remodeler null mutants, and double mutants to observe phenotypic effects and interactions. We further compiled comprehensive nucleosome mapping and RNA sequencing data sets for all spacing remodelers in Dictyostelium. Bioinformatic analysis of these data provide novel insights into remodeler functions, and help to establish a paradigm to explain the relationship between remodeler-mediated chromatin organisation and transcriptional regulation.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:709605 |
| Date | January 2016 |
| Creators | Robinson, Mark |
| Publisher | Cardiff University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://orca.cf.ac.uk/99875/ |
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