Here the use of the in vitro spheroid conformation model, is carried out in such a way as to make use of confocal laser scanning microscopy on vital tissues. Its use in the observation of the invasion of stromal tissues by cancer tissues and the progression of this invasion dynamically was investigated. The employment of simple routine laboratory techniques and the adaptation of this method for the dynamic investigation of human tissues and cancers during the invasion process was paramount. The spheroids for the confrontations were produced from re-aggregates of cells grown in monolayer culture stained with vital fluorescent dyes CMFDA and CMTMR.. The confrontation method seemed appropriate for the examination of invasiveness as it enables the observation of the invasive process on a three dimensional structure which emulates the situation in vivo. The use of the clinically significant parameter INVASLOG is described here and its limits have been elucidated. It has been demonstrated that the use of the INVASLOG enables an objective estimation of the invasive process in vital cultures. Autologous clones of mouse melanoma K-1735, of varying invasive behaviour in vivo and human melanomas A375 and A2058 can be distinguished between in their behaviour. The investigation of the effect of retinoic acid has also demonstrated and the usefulness of this method and the invaslog parameter in investigating invasion. Its use in the investigation of and its application to, diagnosis and prognosis of cancer should be investigated particularly where specific therapies for specific cancers can be tested and the treatment regime adapted for each patient individually.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:583410 |
| Date | January 2004 |
| Creators | DeVaney, Trevor |
| Publisher | Cardiff University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://orca.cf.ac.uk/55373/ |
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