Normal and pathological passaged BMdSCs were successfully grown to confluence over 3 passages and induced to differentiate down the osteogenic and adipogenic lineage with the formation of mineral deposit and multidroplet cell clusters respectively. JMML BMdSCs had a significantly increased growth rate and MDS BMdSCs had a significantly increased number of small colonies in comparison to normal. Lymphoma BMdSCs had an increased CD105, CD49b and CD45 antigenic expression; myeloma BMdSCs had an increased CD105 antigenic expression, and the lymphoproliferative BMdSCs had a significantly reduced CD90, CD105 and CD49b expression in comparison to normal. With osteogenic induction, AML and lymphoma (mineralised) cultures had a reduced osteocalcin gene expression in comparison to normal. With adipogenic induction, AML and lymphoma cultures showed reduced multidroplet cell cluster formation with comparable gene expression to normal.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:583733 |
| Date | January 2006 |
| Creators | Almond, Wendy Rachel |
| Publisher | Cardiff University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://orca.cf.ac.uk/54102/ |
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