Human schistosomiasis is one of the helminthic neglected tropical diseases. It leads to serious health problems and imposes a huge burden on communities. All interactions between the parasite and the human body occur at a molecular and cellular level. Therefore, the study of the molecular aspects of infection and the immune response is a very active area of research. It has been known for decades that there is a direct relationship between protection against infection, or reinfection after treatment, and parasite-specific Immunoglobulin E (IgE) antibody in infected individuals. This study aims to investigate the allergenicity of S. mansoni antigens using RS-ATL8 reporter cell line assay. Towards this goal, I first identified the best reporter cell line for allergenicity assessment. This was done by the characterisation of the transgenic human FcԑRIα chain’s gene copy number and by comparing the levels of human FcԑRI receptor surface expression. The second goal was the optimisation of RS-ATL-8 reporter cell line. This was achieved by the optimisation of fundamental conditions such as the cell density, sensitising agent’s optimum dilution and the stimulant optimum concentration. Once a robust standard operating procedure (SOP) had been established, I investigated the allergenicity of the four expressed S. mansoni antigens using the optimised reporter cell line RS-ATL8.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:748280 |
| Date | January 2018 |
| Creators | Ali, Eman |
| Publisher | University of Nottingham |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://eprints.nottingham.ac.uk/49380/ |
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