This work concerns the identification of the information controlling the accumulation of the influenza virus nucleoprotein (NP) in the nuclei of Xenopus oocytes. The NP accumulates in the nuclei of Xenopus oocytes whether introduced into the oocytes as the protein itself or encoded in RNA or DNA. Since no other influenza virus components are present in the oocytes injected with DNA, this accumulation appears to be a property of the NP itself. In vitro mutagenesis of the cloned NP cDNA has then been used to identify which regions of the protein are important in its nuclear accumulation. Mutant proteins lacking amino acids 327-345 of wild-type NP enter the nucleus but do not accumulate there to the same extent as the wild-type protein, suggesting that this region has a role in nuclear accumulation. This proposed location is strengthened by studies involving the production of fusion proteins in which various amino- terminal sequences of the NP gene are fused to the complete chimpanzee ꙋ1-globin sequence: when globin cDNA is injected into and expressed in oocytes the protein remains in the cytosol, however when the globin cDNA is fused to a portion of NP cDNA which includes the region encoding amino acids 327-345 the resulting fusion protein enters and accumulates in the nucleus. Fusion proteins lacking this region of the NP enter but do not accumulate in the nucleus.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:354723 |
| Date | January 1984 |
| Creators | Davey, John |
| Publisher | University of Warwick |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://wrap.warwick.ac.uk/114391/ |
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