This work describes the development and the optimisation of microfluidic radiolabelling by using an Eckert & Ziegler device remotely controlled by a computer to perform the multistep synthesis of [18F]-fluoro-2-deoxy-D-glucose ([18F]-FDG). This device was then modified to control the fluidic transfers via flow of nitrogen and vacuum with the aid of a new one-way cassette system by using different concentrations of water for the Kryptofix solution. A new route was also explored to perform florbetaben ([18F]-BAY94- 9172), a potent Alzheimer’s disease PET tracer. Chapter 1 is an overview of positron emission tomography techniques to synthesise tracers. Chapter 2 is a brief introduction of the PETIC centre (Wales), where the fluoride-18 was delivered to produce [18F]-FDG. Chapter 3 describes the reaction of the [K+⊂2.2.2]18F− complex on mannose triflate and the variety of products formed. The Eckert & Ziegler platform was furnished with a cassette module and a microfluidic tubing to perform the radiolabelling fluorination of mannose triflate to the tetraacetate [18F]-deoxy-D-glucose and [18F]-FDG. Chapter 4 is focused on the modifications on the Eckert & Ziegler modules by using 1-way cassettes to limit the use of mechanical valves. [18F]-FDG was synthesised via a semiautomated procedure by limiting the number of modules present in the hot cell. Chapter 5 is an investigation for the preparation of several triethylene glycol derivatives following the syntheses of Kryptofix [2.2.2]. The use of triethylene glycol chain will be essential for the synthesis of the Florbetaben precursor. The Chapter 6 is the exploration of a new synthetic route leading to the precursor of florbetaben.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:633532 |
| Date | January 2014 |
| Creators | Marie, Guillaume |
| Publisher | Cardiff University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://orca.cf.ac.uk/67939/ |
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