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Previous issue date: 2016-10-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O estudo para a obten??o de nanopart?culas de ouro (AuNPs) funcionalizadas por mol?culas bioativas tem se mostrado relevante no desenvolvimento de novas alternativas para tratamento do c?ncer. O presente trabalho descreve a s?ntese de AuNPs estabilizadas por dois derivados quinoxal?nicos, 2,3-dietanolaminaquinoxalina (DEQX) e 2-(2,3-di-idro-[1,4]oxazino[2,3-b]quinoxalin-4-il)etanol (OAQX), sintetizados por nosso grupo de pesquisa, verificados por possu?rem atividade antic?ncer em estudos envolvendo c?lulas. Para obten??o das AuNPs funcionalizadas com DEQX e OAQX, o glicerol foi utilizado como principal agente redutor. As AuNPs foram caracterizadas por espectroscopia ultravioleta-vis?vel (UV-Vis), infravermelho (FTIR-ATR) e microscopia eletr?nica de transmiss?o (TEM). Os resultados de UV-Vis indicaram que a melhor condi??o de s?ntese das AuNPs foi quando as propor??es de Au3+ em rela??o aos ligantes org?nicos foi de 1:0,2 e 1:1 para DEQX e OAQX, respectivamente. Nestas condi??es, foram obtidas AuNPs esf?ricas e monodispersas, com dimens?es m?dias de 9,7 e 11,5 nm quando nas estabiliza??es por DEQX e OAQX, respectivamente. O mecanismo de forma??o das part?culas foi proposto com base nos resultados experimentais, e parece envolver uma complexa??o entre Au3+ e os ligantes previamente ? forma??o das AuNPs. O fato das bandas em torno de 525 nm do espectro de UV-Vis, caracter?sticas das AuNPs, tornarem-se mais intensas e com melhor defini??o com o passar do tempo corroboram para essa hip?tese. Foi realizado um estudo relacionado ? estabilidade das AuNPs em fun??o do pH, onde foi verificado que as mesmas se mostraram relativamente est?veis em pH pr?ximo a 7,4. O ligante DEQX foi mais eficiente na estabiliza??o das AuNPs quando comparado ao OAQX. Os resultados obtidos no presente trabalho indicaram que foram obtidas AuNPs esf?ricas monodispersas funcionalizadas com duas esp?cies org?nicas bioativas, abrindo a possibilidade de explora??o destes sistemas para fins medicinais, em especial para o tratamento do c?ncer. / The study on obtainment of gold nanoparticles (AuNPs) functionalized with bioactive molecules have been shown to be relevant in the development of new alternatives for cancer treatment. This study describes the synthesis of AuNPs stabilized by two quinoxaline derivatives, 2,3- diethanolaminequinoxaline (DEQX) and 2-(2,3-di-hydro-[1,4]oxazine[2,3-b]quinoxaline-4-yl) ethanol (OAQX), synthetized by our research group, verified as possessing anticancer activity in studies involving cells. For the obtainment of AuNPs functionalized with DEQX and OAQX, glycerol was used as main reducing agent. The AuNPs were characterized by ultraviolet-visible spectroscopy (UV-Vis), by infrared (FTIR-ATR), and by transmission electronic microscopy (TEM). The results from the UV-Vis showed that the best synthesis condition of AuNPs happened when the proportions of Au3+ in relation to organic ligands were 1:0,2 and 1:1 for DEQX and OAQX, respectively. In those given conditions, monodispersed spherical AuNPs were obtained, with average size of 9.7 and 11.5 nm when stabilized by DEQX and OAQX, respectively. The particles formation mechanism proposed was based on the experimental results, and it appears to involve a complexation between Au3+ and the referred ligands previously to the formation of the AuNPs. The fact that the bands at around 525 nm from the UV-Vis spectrum - characteristics of AuNPs - have become more intense and with better definition over time corroborate to this assumption. A study related to the stability of AuNPs according to the pH was carried out, being thus possible to verify that they showed to be relatively stable at pH near 7,4. The DEQX ligand was more efficient on the stabilization of AuNPs when compared to the OAQX ligand. The results obtained in this work indicated the formation of spherical monodisperse AuNPs functionalized with two bioactive organic species. This leads to the possibility of exploitation of these systems for medical purposes, especially for cancer treatment.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/22373 |
| Date | 31 October 2016 |
| Creators | Silva, Janine de Ara?jo |
| Contributors | 03114939988, http://lattes.cnpq.br/2848745987784319, Pereira, Marcia Rodrigues, 83115951787, http://lattes.cnpq.br/5623374421279476, Silva J?nior, Arnobio Antonio da, 00840406452, http://lattes.cnpq.br/2593509584288129, Kulesza, Joanna Elzbieta, 70134641477, http://lattes.cnpq.br/1568423340359601, Gasparotto, Luiz Henrique da Silva, Menezes, Fabricio Gava |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM QU?MICA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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