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Identify A-to-I editing targets on mRNA of mouse neuron cells

RNA editing by adenosine deamination is catalyzed by members of an enzyme family
known as adenosine deaminases that act on RNA (ADARs). ADARs can change the
structure of RNA by changing an AU base-pair to an IU mismatch. This frequently
modifies the function of the encoded protein, and an emerging theme associated with
A-to-I mRNA editing is that tissues often regulate the ratio of proteins expressed from
edited and unedited mRNAs to fine-tune cellular responses and functions. In mammals,
pre-mRNA of receptor proteins involved in neurotransmission, including serotonin
receptors and glutamate receptors, are edited. Currently, only a limited number of
human ADAR substrates are known, whereas indirect evidence suggests a substantial
fraction of all pre-mRNAs being affected. To identify RNAs containing inosine residues,
this study used a multi step approach; including (1) inosine-specific base cleavage and
RNase T1 digestion, (2) purification of polyA-tailed mRNA, (3) RT w/ T7-polydT
primer, (4) probe synthesis and microarray analysis. Using this method it is possible to
identify novel targets of A to I editing. Approximately 100 genes showed a significant
decrease in two arrays. Future analysis of these targets should reveal the biomedical
significance of A-to-I editing.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0814106-221123
Date14 August 2006
CreatorsLu, Chiu_chin
ContributorsHurng-wern Huang, Chao-neng Tseng, Chien-chih Chiu
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0814106-221123
Rightscampus_withheld, Copyright information available at source archive

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