Cancer is increasingly prevalent globally and influenced by factors such as obesity and smoking. Cancer itself and chemotherapies, like cisplatin, can affect our reproductive organs and increase the risk of involuntary childlessness. In the ovaries there are oocytes that are surrounded by cumulus cells (CC) and the CC provides the oocyte with nutrients like pyruvate, which is important for the oocytes ability to mature. If the oocyte does not receive sufficient amount of nutrients the ovulation may be compromised. The CC are not well studied, and few prior studies have been done specifically to observe the impact of chemotherapies on CC. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is important for the mitochondrial biogenesis in cells. The purpose of the project was to evaluate a method for RNA extraction in CC and observe the impact of cisplatin exposure on the PPARGC1A expression. CCs were aspirated from bovine ovaries and exposed to cisplatin during in vitro maturation. RNA was eluted using the QIAcube and then quality assured. The primer β2 microglobulin (B2M) was used as an endogenous control in the qPCR. The results showed that PPARGC1A was minimally expressed in bovine CC and was inadequate for use when evaluating if cisplatin changes the RNA expression in CC. However, the QIAcube proved to be a suitable method for RNA extraction from bovine CC and B2M showed to be suitable as an endogenous control in bovine CC.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-532725 |
| Date | January 2024 |
| Creators | Lindgren, Agnes |
| Publisher | Uppsala universitet, Institutionen för medicinsk cellbiologi |
| Source Sets | DiVA Archive at Upsalla University |
| Language | Swedish |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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